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Rangeshwaran, R.
- Characterization and Evaluation of Two Indigenous Bacillus thuringiensis Isolates against Helicoverpa armigera Hubner
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PDF Views:194
Authors
R. Rangeshwaran
1,
K. Veenakumari
1,
Pritam Karmakar
1,
K. Ashwitha
1,
G. Sivakumar
1,
Satendar Kumar
1
Affiliations
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 4 (2011), Pagination: 286-293Abstract
Two isolates of Bacillus thuringiensis isolated from dead lepidopteran larvae from a tea garden in Jorhat, Assam and one isolated from soil sample from Rajasthan, obtained in a nationwide screening program showed bipyramidal crystal morphology. These two isolates named as NBAII-BTAS and NBAII-BTG4 were characterized by their high level of toxicity against diamond back moth (Plutella xylostella). The PCR amplification of these two isolates revealed the expected size of the PCR product for cry1Aa, cry1Ab, cry1Ac, cry1E, cry1G, cry1I, and cry2 of 390 bps, 1111bps, 238 bps, 540 bps, 300 bps, 468 bps, and 1170 bps respectively. Purified cry proteins from each of these two cultures were subjected to SDS-PAGE analysis, where, two distinct bands of 130–140 Kda and 65 Kda corresponding to cry1 and cry2 proteins were observed. Toxicity studies was carried out using trypsin activated purified proteins against Helicoverpa armigera, where NBAII-BTG4 derived crystal proteins displayed more toxicity (0.93µg\ml) than NBAII-BTAS.Keywords
Cry Genes, PCR, Specific Cry Primers, Bipyramidal, Toxicity.References
- Aly, N. A. H. 2007. PCR detection of cry genes in local Bacillus thuringiensis Isolates, Australian Journal of Basic and Applied Sciences, 1: 461–466.
- Anitha, D., Senthil Kumar, N., Vijayan, D., Kunhikrishnan., Ajithkumar and Gurusubramanian, G. 2011. Characterization of Bacillus thuringiensis isolates and their differential toxicity against Helicoverpa armigera populations. Journal of Basic Microbiology, 51: 107–114.
- Avilla, C., Vargas-Osuna, E., González-Cabrera, J., Ferré, J. and González-Zamora, J. E. 2005. Toxicity of several ä-endotoxins of Bacillus thuringiensis against Helicoverpa armigera (Lepidoptera: Noctuidae) from Spain. Journal of Invertebrate Pathology, 90: 51–54.
- Avilla. C., Vargas-Osuna, E., González-Cabrera, J., Ferré, J. and González-Zamora, J. E. 2005. Toxicity of several endotoxins of Bacillus thuringiensis against Helicoverpa armigera (Lepidoptera: Noctuidae) from Spain. Journal of Invertebrate Pathology, 90: 51–54.
- Ben-Dov, E., Wang, Q., Zaritsky, A., Manasherob, R., Barak, Z., Schneider, B., Khamraev, A., Baizhanov, M., Glupov, V. and Margalith, Y. 1999. Multiplex PCR screening to detect cry 9 genes in Bacillus thuringiensis strain. Applied and Environmental Microbiology, 65: 3714–3716.
- Beron, C. M., Curatti, L. and Salerno, G. L. 2005. New strategy for identification of novel cry type genes from Bacillus thuringiensis strains. Applied and Environmental Microbiology, 71: 761–765.
- Beron, C. M. and Salerno, G. L. 2006. Characterization of Bacillus thuringiensis isolates from Argentina that are potentially useful in insect pest control. Biological Control, 51: 779–794.
- Bravo A., Sarabia, S., Lopez, L., Ontiveros, H., Abarca, C., Ortiz, A., Ortiz, M., Lina, L., Villalobos, F., Peña, G., Nuñez-Valdez, M. E., Soberón, M and Quintero, R. 1998. Characterization of cry genes in a mexican Bacillus thuringiensis strain collection. Applied and Environmental Microbiology, 64: 4965–4972.
- Carozzi, N. B., Kramer, V. C., Warren, G. W., Evola, S. and Koziel, M. G. 1991. Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Applied and Environmental Microbiology, 57: 3057–3061.
- Cerón, J. L., Covarrubias, R., Quintero, A., Ortiz, M., Ortiz, E. Aranda and Bravo, A. 1994. PCR analysis of the cry I insecticidal crystal family genes from Bacillus thuringiensis. Applied and Environmental Microbiology, 60: 353–356.
- Cerón, J. A., Ortiz, R., Quintero, L. G. and Bravo, A. 1995. Specific PCR primers directed to identify cryI and cry III genes within a Bacillus thuringiensis stray collection. Applied and Environment Microbiology, 61: 3826–3831.
- Chilcott, C. N. and Wigley, P. J. 1994. Isolation and toxicity of Bacillus thuringiensis from soil and insect habitats in New Zealand. Journal of Invertebrate Pathology, 61: 244–247.
- Gill, S. S., Cowles, E. A. and Pietrantinio, P. V. 1992. The mode of action of Bacillus thuringiensis endotoxins. Annual Review of Entomology, 37: 615–636.
- Höfte H. and Whiteley H. R., 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiological Reviews, 53: 242–255.
- Hofte, H., Van Rie, J., Jansens, S., Van Houtven, A., Vanderbruggen, H. and Vaeck, M. 1988. Monoclonal antibody analysis and insecticidal spectrum of three types of lepidopteran specific insecticidal crystal proteins of Bacillus thuringiensis. Applied and Environmental Microbiology, 54: 2010–2017.
- Konecka, E., Kaznowski, A., Zeimnicka, J., Zeminicki, K. and Paetz, H. 2007. Analysis of cry gene profiles in Bacillus thuringiensis strains isolated during epizootics in Cydia pomonella L., Current Microbiology, 55: 217–222.
- Kronstad, J. W. and Whiteley, H. R. 1986. Three classes of homologous Bacillus thuringiensis crystal protein genes. Gene, 43: 29–40.
- Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 227: 680–685.
- Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. Journal of Biology and Chemistry, 193: 26.
- Mahadi, N. M., Hastowo, S., Lay, B. and Dean, D. H. 1998. Application of Multiplex P.C.R. for rapid determination of cryI gene profiles of new Bacillus thyringiensis isolates. Journal of Microbiology and Biotechnology, 8: 517–522.
- Martin, P. A. W., Travers, R. S. 1989. Worldwide abundance and distrbution of Bacillus thuringiensis isolates. Applied Environmental Microbiology, 55: 2437–2442.
- Morris, O. N., Converse, V., Kanagaratnam, P. and Cote, J. C. 1998. Isolation, characterization and culture of Bacillus thuringiensis from soil and dust from grain storage bins and their toxicity for Mamestra configurata (Lepidoptera: Noctuidae). Canadian Entomologist, 130: 515–537.
- Obeidat, M., Hassawi, D. and Ghabeish I. 2004. Characterization of Bacillus thuringiensis strains from Jordan and their toxicity to the Lepidoptera Ephestia kuehniella Zeller. African Journal of Biotechnology, 3: 622–626.
- Ohba, M. and Aizawa, K. 1986. Distribution of Bacillus thuringiensis in soils of Japan. Journal of Invertebrate Pathology, 47: 277–282.
- Porcar, M. and Jua.rez/Perez, V. 2003. PCR-based identification of Bacillus thuringiensis pesticidal crystal geners. FEMS Microbiology Reviews, 26: 419–432.
- Rosas-Garcia, N. M., Mireles-Martinez, M., Hernandez-Mendoza, J. L. and Ibarra, J. E. 2007. Screening of cry gene contents of Bacillus thuringiensis strains isolated from avocado orchards in Mexico, and their insecticidal activity towards Argyrotaenia sp. (Lepidoptera:Tortricidae) larvae. Journal of Applied Microbiology, 104: 224–230.
- Sambrook, J., Fritsch, E. F. and Mariatis, T. 1989. Molecular cloning: A Laboratory Manual, 2nd Ed., N. Y., Cold Spring Harbor Laboratory Press, 659p.
- Smith, R. A. and Couche, G. A. 1991. The phylloplane as a source of Bacillus thuringiensis. Applied and Environmental Microbiology, 57: 311–315.
- Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Fietelson, J., Ziegler, D. R. and Dean, D. H. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiology and Molecular Biology Reviews. 62: 775–806.
- Shang Kuo, W., Lin, J. H., Tzeng, C. C., Kao, S. S. and Chak, K. F. 2000. Cloning of two new cry Genes from Bacillus thuringiensis subsp. wuhanensis strain. Current Microbiology, 40: 227–232.
- Thammasittirong, A. and Attathom, T. 2008. PCR-based method for the detection of cry genes inlocal isolates of Bacillus thuringiensis from Thailand. Journal Invertebrate Pathology, 98: 121–126.
- Travers, R. S., Martin, P. A. and Reichelderfer, C. F. 1987. Selective process for efficient isolation of soil Bacillus spp. Applied and Environmental Microbiology, 53: 1263- 1266
- ITS Sequencing of Indian Isolates of Lecanicillium Species
Abstract Views :212 |
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 4 (2011), Pagination: 337-341Abstract
The genetic diversity of thirty one isolates of Lecanicillium species isolated from insect hosts from various geographical regions of India were studied. Their phylogenetic relationships were determined using internal transcribed spacer, ITS1, ITS2 and 5.8S gene of rRNA sequence. Based on the sequence similarity of ITS region and construction of subsequent phylogenetic analysis (neighbour-joining method), 15 isolates were grouped as Lecanicillium lecanii, 11 isolates as L. attenuatum, 3 isolates as L. longisporum and 2 isolates as L. muscarium.Keywords
ITS Region, Phylogenetic Analysis, Lecanicillium sp.References
- Aiyar, A. 2000. The use of Clustal W and Clustal X for multiple sequence alignment. Methods in Molecular Biology, 132: 221–241.
- Altschul, S. F., Gish, W., Miller, W., Myers, E. W. and Lipman, D. J. 1990. Basic local alignment search tool. Journal of Molecular Biology, 215: 403–10.
- Boysen, M., Skouboe, P., Frisvad, J. and Rossen, L. 1996. Reclassification of the Penicillium roqueforti group into three species on the basis of molecular genetic and biochemical profiles. Microbiology, 142: 541–549.
- Carder, J. H. and Barbara, D. J. 1999. Taxonomic status of putative Verticillium albo-atrum isolates. FEMS Microbiology Letters, 170: 211–219.
- Gams, W. and Zare, R. 2001. A revision of Verticillium sect. Prostrata. III. Generic classification. Nova Hedwigia, 72: 47–55.
- Hall, R. A. 1981. Laboratory studies on the effects of fungicides, acaricides and insecticides on the entomopathogenic fungus, Verticillium lecanii. Entomologia Experimentalis et Applicata, 29: 39–48.
- Steenberg, T. and Humber, R. A. 1999. Entomopathogenic potential of Verticillium and Acremonium species (Deuteromycotina: Hyphomycetes), Journal of Invertebrate Patholology, 73: 309–314.
- Sugimoto, M., Koike, M., Hiyama. N. and Nagao, H. 2003. Genetic, morphological and virulence characterization of the entomopathogenic fungus Verticillium lecanii. Journal of Invertebrate Pathology, 82: 176–187.
- Sung, G. H., Zare, R., Spatafora, J. and Gams, W. 2001. Verticillium section Prostrata. 11. Generic delimitation using ribosomal DNA sequences. Nova Hedwigia, 72: 29–46.
- Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choice. Nucleic Acids Research, 22: 4673–4680.
- White, T. J., Bruns, T. D., Lee, S. B. and Taylor, J. W. 1990. Amplication and direct sequencing of fungal ribosomal RNA genes for phylogenetics. pp. 315–322. In: Innis M.A., Gelgand, D.H., Suinsky, J.J. and White, J.J., (Eds.), PCR protocols: A Guide to Methods and Applications, Academic Press, San Diego.
- Zare, R. and Gams, W. 2001. A revision of Verticillium section Prostrata. IV. The genera Lecanicillium and Simplicillium gen. nov. Nova Hedwigia, 73: 1–50.
- Zare, R. and Gams, W. 2004. A monograph of Verticillium section Prostata. Rostaniha, 3: 1–188.
- Zare, R., Gams, W. and Culham, A. 2000. A revision of Verticillium sect. Prostrata. 1. Phylogenetic studies using ITS sequences. Nova Hedwigia, 71: 465–480.
- Zare, R., Gams. W. and Evans, H. C. 2001. A revision of Verticillium section Prostrata. V. The genus Pochonia, with notes on Rotiferophthora. Nova Hedwigia, 73: 51–86.
- Relationship between Indole Acetic Acid Production by Fluorescent Pseudomonas and Plant Growth Promotion
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Authors
Affiliations
1 Nallamuthu Gounder Mahalingam College, Pollachi, 642 001, Coimbatore, Tamil Nadu, IN
2 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore, 560 024, Karnataka, IN
1 Nallamuthu Gounder Mahalingam College, Pollachi, 642 001, Coimbatore, Tamil Nadu, IN
2 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore, 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 24, No 4 (2010), Pagination: 349-359Abstract
Forty fluorescent pseudomonads were quantitatively evaluated for indole acetic acid (IAA) producing ability in the presence (trypt+) or absence (trypt-) of tryptophan and growth promotion in groundnut in response to seed treatment with high IAA producers was analyzed. There were significant differences in the amounts of IAA produced by the isolates and more amounts of IAA were released in trypt+. In trypt- increased IAA production was noticed for up to 144h and thereafter it stabilized or decreased. Analysis of plant growth promotion showed that the maximum ischolar_main length (187.5mm) was exhibited by plants treated with BA16(A)2 which was a medium IAA producer whereas the highest IAA producer BA1(E)2 (32.2 μg/ml of IAA) showed a ischolar_main length of 167.3mm. The isolate OTN7(E)2 which was a low IAA producer showed good ischolar_main lengths of 148.3mm (sterile soil) and 133.3mm (unsterile soil). When the shoot lengths were compared, highest shoot length of 213.3mm (sterile soil) was by BA4(D) which is a medium to high IAA producer and in unsterile soil highest shoot length of 198.67mm was shown by the isolate ND1which is also a medium to high IAA producer. The highest IAA producer BA1(E)2 showed a low shoot length of 156.7mm (sterile soil) and 115mm (unsterile soil). The results in some way do establish that plant growth is not directly influenced by high IAA producers. Root growth seemed to be more affected by high IAA producers in unsterile soil and maximum vigour index of 32600 was exhibited by BA2(D)1 which is a medium IAA producer and the minimum of 15750 was by BA1(E)2 which is a high IAA producer. Hence IAA producers of the fluorescent Pseudomonas group showed significant plant growth when compared with control but plant growth was not greatly influenced by those organisms that produced high amounts of IAA. Antagonistic Pseudomonas spp. able to release moderate or even low amounts of IAA may be better growth promoters.Keywords
Pseudomonas, Indole Acetic Acid, Shoot Length, Root Length, Plant Growth Promotion, Tryptophan, Vigour Index.- Screening and Identification of Potential Bacillus Spp. for the Management of Bacterial Wilt of Brinjal
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, H.A. Farm Post, Hebbal, Bellary Road, Bangalore, 560024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, H.A. Farm Post, Hebbal, Bellary Road, Bangalore, 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 3 (2011), Pagination: 229-235Abstract
Among 100 isolates of Bacillus spp. screened in vitro against Ralstonia solanacearum that causes bacterial wilt of brinjal, ten were found inhibitory to R. solanacearum. Bacillus megaterium isolate NBAII 63 was highly inhibitory with 29.20 mm of inhibition zone against R. solanacearum as compared to the other nine strains of Bacillus. Six of them were identified by 16S rDNA analysis. The bioefficacy of talc formulation of B. megaterium was evaluated under greenhouse for plant growth promotion and suppression of bacterial wilt in brinjal. The bacterial wilt was effectively managed by B. megaterium through different methods of applications. A combination of four methods (seed treatment + soil application + seedling ischolar_main dip + foliar spray) was the most effective. Maximum ischolar_main length (23.42 cm), shoot length (65.21 cm), fresh weight (40.39 g), dry weight (10.33 g) and highest wilt reduction (50.54%) was recorded in the combination method. Among single application methods, seed treatment was effective exhibiting 41% reduction of bacterial wilt followed by soil application which gave 36% wilt reduction. The bacterial wilt reduction in chemical control (streptomycin sulphate) was 71%. Good growth of the brinjal plants was recorded due to application of B. megaterium. Highest rhizosphere population of 67.0 × 106 cfu/g was recorded in brinjal at 40 days after transplanting when the antagonist was applied by combining the different application methods.Keywords
Bacillus Spp, Ralstonia solanacearum, Bioefficacy, Talc Formulation, Brinjal.References
- Alam, S., Khalil, S., Ayub, N. and Rashid, M. 2002. In vitro solubilization of inorganic phosphate by phosphate solubilising microorganism (PSM) from maize rhizosphere, International Journal of Agricultural Biology, 4: 454–458. Brooks, S. D., Gonzalez, C. F., Appel, D. N. and Filer, T. H. 1994. Evaluation of endophytic bacteria as potential biological control agents for oak wilt. Biological Control, 4: 373– 381.
- Bloemberg, G. V. and Lugtenberg, B. J. J.2001. Molecular basis of plant growth promotion and biocontrol by rhizobacteria. Current opinion in Plant Biology, 4: 343–350.
- Dhingra, O. D. and Sinclair J. B. 1995. In: Basic Plant Pathology Methods, 2nd Ed. CRC Press, Boca Raton, FL, USA.
- Doan T. T. and Nguyen, T. H. 2006. Status of research on biological control of tomato and groundnut bacterial wilt in Vietnam, pp. 105–111. In: Wolfgang Zeller, Cornelia Ulrich (eds.), 1st International Symposium on Blogical Control of Bacterial Plant Diseases, Darmstadt, Germany, 2005.
- Grimault, P. V., Schmit, J. and Prior, P. 1993. Some characteristics involved in bacteria resistance in tomato, pp. 112–119. In: Hartman, G. L. and A. C. Hayward (eds.), Bacterial wilt. ACIAR proceedings No. 45: Australian centre for International Agriculture Research, Camera.
- Han, H. S. and Lee, K. D. 2005. Phosphate and potassium solubilising bacteria effect on mineral uptake, soil availability and growth of eggplant. Researh Journal of Agriculture and Biological Sciences, 1: 176–180.
- Han, H. S., Lee, S. and K. D. 2006. Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant and Soil Environment, 52: 130–136.
- Higgins, D. G., Bleashy, A. T. and Fuchs, R. 1992. Clustal V: Improved software for multiple sequence alignment. Computer Application in Biosciences, 8: 189–191.
- Jung, H. K. and Kim, S. D. 2005. An antifungal antibiotic purified from Bacillus megaterium KL 39, a biocontrol agent of red-pepper Phytophthora blight disease. Journal of Microbiology and Biotechnology, 15: 1001-1010.
- Kelman, A. 1954. The relationship of pathogenicity in Pseudomonas solanacearum colony appearance on a tetrazolium medium. Phytopathology, 44: 693–696.
- Kloepper, J. W. 1997. Current status and future trends in biocontrol research and development in the U S, pp. 49– 52. In: Proceedings of the International Symposium of Clean Agriculture, Japan.
- Liu, Z. L. and Sinclair, J. B., 1992. Population dynamics of Bacillus megaterium strain B153–2-2 in the rhizosphere of soybean. Phytopathology, 82: 1297–1301.
- Lwin, M. and Ranamukhaarachchi, S. L. 2006. Development of biological control of Ralstonia solanacearum through antagonistic microbial populations. International Journal of Agriculture and Biology, 8: 657–660.
- McSpadden – Gardener, B. B., and Fravel, D. R. 2002. Biological control of plant pathogens. In: Research, commercialization and application in the USA. Online. Plant Health Progress, doi:10.1094/PHP-2002-0510-01-RV.
- Norris J. R., Berkeley, R. C. W., Logan, N. A. and O’Donnell, A. G. 1981. The genera Bacillus and Sporolactobacillus, pp. 1711–42. In: Star M. P., Stolp, A., Truper, A. G., Balows, A. and Schlegel, H. G, (eds). The Prokaryotes, vol. 2. Berlin: Springer.
- Nguyen, M. T. and Ranamukhaarachchi, S. L. 2010. Soil borne antagonists for biological control of bacterial wilt caused by Ralstonia solanacearum in tomato and capsicum. Journal of Plant Pathology, 92: 385–395.
- Nguyen, M. T., Ranamukhaarachchi, S. L. and Hannaway, D. B. 2011. Efficacy of Antagonist Strains of Bacillus megaterium, Enterobacter cloacae, Pichia guilliermondii and Candida ethanolica against Bacterial Wilt Disease of Tomato. Journal of Phytology, 3: 01–10.
- Rangeshwaran, R., Vajid, N. V., Ramanujam, B., Sriram, S., Bhaskaran, T. V. and Kumar, S. 2010. Additives in powder based formulation for enhanced shelf life of Pseudomonas fluorescens and Bacillus spp. Journal of Biological Control, 24: 158–163.
- Roberts, T. and Hitchins, A.1969. Resistance of spores, pp. 611– 671. In: Gould, G. W. and Hurst, A. (Eds.), The Bacterial Spore. Academic Press, London UK,.
- Sally K. Mathew and Peter, K. V. 2004. Bacterial wilt in warm humid tropics of Kerala, 24 pp. Kerala Agricultural University, Vellanikkara, Thrissur, India.
- Sneath, P. H. A. 1986. Endospore-forming Gram-positive rods and cocci, pp 1104-1109. In: Sneath P. H. A., Nair N. S., Sharpe M. E. and Holt J. G. (eds), Bergeys manual of systematic bacteriology. Vol. 2. 9th ed. Baltimore, M. D. Willaims and Wilkins..
- Shekhawat, G. S., Chakrabarti, S. K., Kishore V., Sunaina V. and Gadewar A. V. 1993. Possibilities of biological management of potato bacterial wilt with strains of Bacillus sp., B. subtilis, Pseudomonas fluorescens and Actinomycetes, pp. 327–330. In: Hartman G. H. and Hayward,A.C. (eds.). Bacterial wilt, Australian Center for International Agricultural Research, Canberra.
- Sivaprasad, P. 2002. Microbial inoculant technology for plant disease management, pp. 23–30. In: Research Extension interface, Farm Information bureau. Government of Kerala.
- Subbarao, N. S. 1988. Phosphate solubilizing micro-organism, pp. 133–142 In: Biofertilizer in agriculture and forestry. Regional Biofertilizer Development Centre, Hissar, India.
- Nishijima, T., Toyota, K. and Mochizuki, M. 2005. Predominant culturable Bacillus species in Japanese arable soils and their potential as biocontrol agents. Microbes and Environmet, 20: 61–68.
- Weller, D. M. 1998. Biological control of soil borne plant pathogens in the rhizoshere with bacteria. Annual Review of Phytopathology, 26: 379–407
- Winstead, N. N. and Kelman, A. 1952. Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology, 42: 628–634.
- Wu, S. C., Cao, Z. H., Li, Z. G, Cheung, K. C. and Wong, M. H. 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma, 125: 155–166.
- Wydra, K. and Semrau J., 2005. Phenotypic and molecular characterization of the interaction of antagonistic bacteria with Ralstonia solanacearum causing tomato bacterial wilt, pp. 112-118, In: Zeller W. And Ulrich C. (eds). 1st International Symposium on Biological Control of Bacterial Plant Diseases. Darmstadt, Germany.
- Chitinase Activity and Virulence of Different Isolates of Beauveria bassiana, Metarhizium anisopliae and Lecanicillium Spp
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 3 (2011), Pagination: 223-228Abstract
In order to identify promising isolates with higher chitinase activity, 122 entomofungal isolates comprising of Beauveria bassiana (58), Metarhizium anisopliae (33), Lecanicillium lecanii (15), Lecanicillium attenuatum (11), L. longisporum (3) and L. muscarium (2) isolated from different insect hosts/soils of India were studied. The partially purified proteins from the isolates were subjected to chitinase activity and were estimated by measuring the release of reducing saccharides (one NAGA Unit) from colloidal chitin spectrophotometrically at 582 nm (A582). Forty nine isolates of B. bassiana showed chitinase activity ranging from 21 to 182 μg/ml, with the highest enzyme activity by the isolate PDBC-Bb-5a. Thirty three isolates of M. anisopliae exhibited chitinase activity ranging from 23 to 144 μg/ml and the highest (144 μg/ml) was by the isolate Ma-4. Among the 15 isolates of L. lecanii tested, three isolates viz., Vl-7, Vl-24a, Vl-25a had high chitinase activities ranging between 100 and 126μg/ml. Vl-22 isolate of L. attenuatum, Vl-24 of L. longisporum and Vl-8 of L. muscarium showed higher activities (90, 110 and 117μg/ml respectively). Bioassay studies with these isolates on cowpea aphid, Aphis craccivora in glass house indicated higher nymphal mycosis ranging from 72.3-83.0%.Keywords
Entomopathogenic Fungi, Chitinase Activity, NAGA Unit, Cowpea Aphid, Aphis craccivora, Virulence.References
- Charnley, A. K. 1984. Physiological aspects of destructive pathogenesis in insects by fungi; a speculative review. In: J. M. Anderson, A. D. M. Rayner and D. W. H. Walton (eds) Invertebrate-Microbial Interactions, pp. 229–270. Cambridge University Press.
- Havukkala, I., Mitamura, C., Hara, S., Hirayae, K., Nishizawa, Y. and Hibi, T. 1993. Induction and purification of Beauveria bassiana chitinolytic enzymes. Journal Invertebrate Pathology, 61: 97– 102.
- Hepburn, H. R. 1985. Structure of the integument. In Kerbut, G.A. and Gilbert, L.I, (eds) Comprehensive Insect Physiology, Biochemistry and Pharmacology. Volume, 3, pp. 1–58. Pergamon Press, Oxford, UK. Krieger de Moraes, C., Schrank, A. and Vainstein, M. H., 2003. Regulation of extracellular chitinase and proteases in the entomopathogen and acaricide Metarhizium anisopliae. Current Microbiology, 46: 205–210.
- Nahar, P., Ghormade, V. and Deshpande, M.V. 2004. The extracellular constitutive production of chitin deacetylase in Metarhizium anisopliae: Possible edge to entomopathogenic fungi in the biological control of insect pests. Journal of Invertebrate Pathology, 85: 80–88
- Samuels, K. D. Z., Heale, J. B. and Llewellyn, M. 1989. Characteristics relating to the pathogenicity of Metarhizium anisopliae toward Nilaparvata lugens. Journal of Invertebrate Pathology, 53: 25–31.
- St. Leger, R. J., Cooper, R. M. and Charnley, A. K. 1991. Characterization of chitinase and chitobiase produced by the entomopathogenic fungus Metarhizium anisopliae. Journal of Invertebrate Pathology, 58:415–426.
- Yanai, K., Takaya, N., Kojima, M., Horiuchi, H., Ohta, A. and Takaki, M. 1992. Purication of two chitinases from Rhizopus oligosporus and isolation and sequencing of the encoding genes. Journal of Bacteriology, 174: 7398–7406.
- Additives in Powder Based formulation for Enhanced Shelf Life of Pseudomonas fluorescens and Bacillus sp.
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 24, No 2 (2010), Pagination: 158-163Abstract
Studies were carried out to evaluate the effect of adding nutrient additives to talc based formulations on the shelf life of bacteria stored under room temperature. Two organisms namely, Pseudomonas fluorescens (PDBCAB2) and Bacillus sp. (MTCC6534 - chickpea endophyte), a spore forming bacterium, were tested. A gradual increase in P. fluorescens population up to 90 days was observed in almost all the treatments that were amended with nutrients. At 90 days highest population of log 10.38 cfu g−1 was noticed in talc amended with 2 per cent tryptone and glycerol. Decline in the population was observed from 90 days onwards and was rapid from 150 days onwards. At 240 days highest count of log 1 x 104.2 cfu g−1 was obtained with formulations amended with 2% tryptone and 2% glycerol and the results indicated that 2% peptone or 2% tryptone supplemented with 2% glycerol helped P. fluorescens in its better survival. The spore forming Bacillus sp. survived well throughout the study period. At 240 days all talc formulations amended with nutrient sources showed a population of log 9.0 cfu g−1 or above and the population declined to below log 7.0 cfu g−1 only in non amended treatments. Slow decline started from 240 days but high cfu of log 9.30 to 9.38 g−1 were noticed in yeast extract or tryptone treated treatments. Hence talc formulations that had yeast extract or tryptone supplemented with glycerol enhanced shelf life of Bacillus spp.Keywords
Pseudomonas fluorescens, Bacillus sp, Powder Based Formulation, Talc, Shelf Life, Gram Positive, Gram Negative.- Post Harvest Fruit Bioassay of Phylloplane, Pomoplane and Endophytic Microbes against Chilli Anthracnose Pathogen, Colletotrichum capsici (Syd.) E. J. Butler&bisby
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, HA Farm post, Bellary Road, Hebbal, Bangalore 560024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, HA Farm post, Bellary Road, Hebbal, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 26, No 1 (2012), Pagination: 62-69Abstract
Two hundred and fifty eight phylloplane/endophytic bacterial isolated from chilli leaves/fruits and one hundred pomoplane yeast isolates from vegetable/fruits were screened against Colletotrichum capsici by fruit bioassay (post harvest) method. Among the pomoplane bacterial isolates tested, Bacillus tequilensis (PMB-185) gave highest reduction (67.84%) of lesion development, where as among the phylloplane bacterial isolates, PHB-25 exhibited highest (48.65%) suppression of lesion caused by C. capsici. Among the endophytes tested, B. megaterium (ENB-86) produced the highest suppression of lesion (59.66%) and rhizospheric bacterium Pseudomonas putida (PBA-5) showed 50.68% suppression. Six bacteria exhibiting significant suppression (50.29 to 67.84%) were identified by 16s rDNA analysis and all of them belonged to Bacillus spp. including B. tequilensis (PMB-185), B. pumilus (PMB- 183), two B. subtilis (PMB-123 and ENB-24) and two B. megaterium (PMB-53 and ENB-86). Among the yeast isolates tested, the maximum reduction (72.16%) of lesion development was observed with the yeast isolate, Hanseniaspora uvarum (Y-73) which was the highest among all the antagonists tested. The results indicated that spraying of H. uvarum (Y-73) or B. tequilensis (PMB-185) on freshly harvested chilli fruits reduced post harvest fruit damage by C. capsici in chilli.Keywords
Chilli Anthracnose, Colletotrichum capsici, Fruit Bioassay, Pichia guilliermondii and Bacillus Species.References
- Chanchaichaovivat A, Ruenwongsa P, Panijpan B. 2007. Screening and identification of yeast strains from fruits and vegetables: Potential for biological control of postharvest chilli anthracnose (Colletotrichum capsici). Biol Control. 42: 326–335.
- Chowdhary A, Ahmad S, Khan ZU, Doval DC, Randhawa HS. 2004. Trichosporon asahii as an emerging etiologic agent of sisseminated trichosporonosis: a case report and an update. Ind J Med Microbiol. 22: 16–22.
- Havenga W, De Jager ES, Korsten L. 1999. Factors affecting biocontrol efficacy of Bacillus subtilis against Colletotrichum gloeosporioides. Sou Afr Avo Gros’ Asso Yearbook. 1999, 22: 12–20.
- Hierro N, Gonzalez A, Mas A, Guillamo JM. 2004. New PCR-based methods for yeast identification J Appl insert Microbiol. 97: 792–80.
- Kloepper JW, Ryu CM, Zhang S. 2004. Inducedsystemic resistance and promotion of plant growth by Bacillus spp. Phytopathology. 94: 1259–66.
- Korsten L, Bezuidenhout JJ Kotzé JM. 1988. Biological control of avocado postharvest diseases. Sou Afr Avo Gros’ Asso Yearbook. 11: 75.
- Korsten L, De Jager, E S. 1995. Mode of action of Bacillus subtilis for control of avocado post-harvest pathogens. Sou Afr Avo Gros’ Asso Yearbook. 18: 124–130.
- Korsten L, De Jager ES, De Villiers EE, Lourens A, Kotze JM, Wehner FC. 1995. Evaluation of bacterial epiphytes isolated from avocado leaf and fruit surfaces for biocontrol of avocado post-harvest diseases. Plant Dis. 79: 1149–1156.
- Korsten L, De Villiers EE, Rowell A, Kotze JM. 1993. Postharvest biological control of avocado fruit diseases. Sou Afr Avo Gros’ Asso Yearbook. 16: 65–69.
- Liu HM, Guo JH, Luo L, Liu P, Wang BQ, Cheng YJ, Deng BX, Long CA. 2010. Improvement of Hanseniaspora uvarum biocontrol activity against gray mold by the addition of ammonium molybdate and the possible mechanisms involved. Crop Prot. 29: 277–282.
- Manandhar JB, Hartman GL, Wang TC. 1995. Anthracnose development on pepper fruits inoculated with Colletotrichum gloeosporioides. Plant Dis. 79: 380–383.
- McInroy JA, Kloepper JW. 1995. Population dynamics of endophytic bacteria in field grown sweet corn and cotton. Can J Microbiol. 41: 895–901.
- Montri P, Taylor PWJ, Mongkolporn O. 2009. Pathotypes of Colletotrichum capsici, the Causal Agent of Chili Anthracnose,in Thailand. Plant Dis. 93: 17–20.
- Ngullie M, Daiho L, Upadhyay DN. 2010. Biological management of fruit rot in the world’s hottest chilli (Capsicum chinense Jacq.). J Pl Prot Res. 50: 269–273.
- Ramamoorthy V, Samiyappan R. 2001. Induction of defense related genes in Pseudomonas fluorescens treated chilli plants in response to infection by Colletotrichum capsici. J Mycol Pl Pathol. 31: 146–155.
- Ramanujam B. 2008, Isolation, Identification and evaluation of biocontrol agents. In: Eapen SJ, Kumar A, Anandaraj M. (Eds.). Plant Pathogens and their Biocontrol agents Diagnostics and Characterization: Indian Institute of Spices Research. p. 207–224.
- Schreiner K, Hagn A, Kyselkova M, Moenne LY, Welzl G, Munch JC, Schloter M. 2010. Comparison of Barley Succession and Take-All Disease as Environmental Factors Shaping the Rhizobacterial Community during Take-All Decline. Appl Environ Microbiol. 76: 4703–4712.
- Stepanovic S, Dakic I, Morrison D, Hauschild T, Jezek P, Petras P, Marte A, Vukovic D, Shittu A, Devriese LA. 2005. Identification and characterization of Clinical isolates of Members of the Staphylococcus sciuri Group. J Clin Microbiol. 43: 956–958.
- Shawn R. Lockhart, Shawn A. Messer, Michael A. Pfaller and Daniel J. Diekema. 2008. Lodderomyces elongisporus Masquerading as Candida parapsilosis as a Cause of Bloodstream Infections. J Clin Microbiol. 46: 374–376.
- Taj ASJ, Doiphode SH, Han XY. 2006. Kodamaea (Pichia) ohmeri fungaemia in a premature neonate. J Med Microbiol. 55: 237–239.
- Tavanti A, Lambert A, Hensgens M, Ghelardi E, Campa M, Senes S. 2007. Genotyping of Candida orthopsilosis Clinical Isolates by amplification fragment length polymorphism Reveals Genetic Diversity among Independent Isolates and Strain Maintenance within Patients. J Clin Microbiol.45: 1455–1462.
- Than PP, Jeewon R, Hyde KD, Pongsupasamit S, Mongkolporn O Taylor, PWJ. 2008. Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathol. 57: 562–572.
- Thind TS, Jhooty JS. 1985. Relative prevalence of fungal diseases of chilli fruits in Punjab. Ind J Mycol Plant Pathol. 15: 305–307.
- Vinaya H, Rao M SL, Yashoda H, Mohankumar HD. 2009. Status of seed borne incidence of anthracnose of chilli in northern Karnataka and evaluation of seed health testing methods for the detection of Colletotrichum capsici. Kar J Agri Sci. 22: 807–809.
- Xue-qing CAI, Hong HE, Yi-yuan YE, Fang-ping HU. 2004.Effect of endophytic bacteria BS-1 and BS-2 (Bacillus subtilis) on the control of capsicum anthrancnose and active oxygen metabolism in capsicum fruits. J Fug Agri Uni. 1: 102–105.
- Evaluation of Diacetylphloroglucinol Producing Pseudomonads for Their Biocontrol Potential Against Ralstonia Wilt in Brinjal
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, P.B. No. 2941, H.A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, IN
2 Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore 560 089, IN
1 National Bureau of Agriculturally Important Insects, P.B. No. 2941, H.A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, IN
2 Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore 560 089, IN
Source
Journal of Biological Control, Vol 27, No 2 (2013), Pagination: 105–109Abstract
Diacetylphloroglucinol (DAPG) producing isolates of pseudomonads were screened against bacterial wilt in brinjal caused by Ralstonia solanacearum under greenhouse conditions. Thirty Pseudomonas isolates were obtained from the culture collection of NBAII, Bangalore that include seven isolates of Pseudomonas putida (CK8C, CK24E, Pf4K, RPF9, RPF13, OTN5E2, GR1ARS1), one P. mosselli (CK24C) isolate, three isolates of P. fluorescens (GR3ARS3, Pf-DWD, CHAO), three isolates of P. plecoglossicida (BA11D1, BA16-2, BA3-D1) and sixteen isolates of P. aeruginosa (CK13C, CK19E, AFP3, AFP4, AFP6, RFP7, OTN8, AFP9, AFP8, PDB8, PDB1, AFP7, AFP5, AFP13, ND4-IARIB, RPF8). Bacterial wilt susceptible brinjal variety MEBH- 9 was used in the screening. The brinjal seedlings were dipped in Pseudomonas suspension for five minutes before transplantation and plants were observed for wilt symptoms like stunted growth, drooping of leaves, loss of rigidity and eventually death of the plant. Four weeks later, the plants were upischolar_mained and length and weight of both ischolar_mains and shoots were recorded. The highest percentage of wilt disease reduction was observed in seedlings treated with P. plecoglossicida BA11D1 (95.8%), P. putida CK24E (62.5%) and P. plecoglossicida BA3D1 (41.7%). Average shoot length, ischolar_main length and shoot weight, ischolar_main weight were comparatively lesser in most of the pseudomonads treated plants than the control plants.Keywords
Ralstonia solanacearum, Bacterial Wilt, Plant Growth Promotion, Pseudomonads, Diacetylphloroglucinol.References
- Bora, LC, Deka, SN. 2007. Wilt disease suppression and yield enhancement in tomato (Lycopersicon esculentum) by application of Pseudomonas fluorescens based biopesticide (Biofor-Pf) in Assam. Indian J Agric Sci. 77: 490–494.
- Guo JH, Qi HY, Guo YH, Ge HL, Gong LY, Zhang LX, Sun PH. 2004. Biocontrol of tomato wilt by plant growthpromoting rhizobacteria. Biol Control 29: 66–72.
- Hayward AC. 1991. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annual Rev Phytopathol. 29: 65–87.
- Evaluation of Ethiopian isolates of Pseudomonas fluorescens as biocontrol agent against potato bacterial wilt caused by Ralstonia (Pseudomonas) solanacearum. Acta Agric Slovenica 90: 125–135.
- Kumar MKP, Khan ANA, Reddy CNL, Basavarajappa MP, Venkataravanappa V, Basha Z. 2006. Biological control of bacterial wilt of tomato caused by Ralstonia solanacearum, race-1, biovar-III. J Pl Dis Sci. 1: 176–181.
- Lemessa F, Zeller W. 2007. Screening rhizobacteria for biological control of Ralstonia solanacearum in Ethiopia. Biol Control 42: 336–344.
- Mahmoud SM. 2007. Management of brown rot disease of potato. Arab Univ J Agric Sci. 15: 457–463.
- Ramesh R, Joshi AA, Ghanekar MP. 2009. Pseudomonads: major antagonistic endophytic bacteria to suppress bacterial wilt pathogen, Ralstonia solanacearum in the eggplant (Solanum melongena L.). World J Microbiol Biotech. 25: 1, 47–55.
- Ran LX, Liu CY, Wub CJ, Van loon LC, Bakker PAHM. 2005. Suppression of bacterial wilt in Eucalyptus urophylla by fluorescent Pseudomonas spp. in China. Biol Control 32: 111–120.
- Smith EF. 1896. A bacterial disease of the tomato, eggplant and Irish potato (Bacillus solanacearum nov. sp.) USDA, Division of Vegetables Physiology & Pathology, Bulletin 12: 28 pp.
- Vanitha SC, Niranjana SR, Mortensen CN, Umesha S. 2009. Bacterial wilt of tomato in Karnataka and its management by Pseudomonas fluorescens. BioControl. 54: 5, 685–695.
- Yabuuchi E, Kosako Y, Yano I, Hota H, Nishiuchi Y. 1995. Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov., Ralstonia solanacearum (Smith, 1986). comb. nov. Microbial Immunol. 39: 897–904.
- Induced Defense Response in Brinjal Plants By Bacillus megaterium NBAII 63 Against Bacterial Wilt Pathogen, Ralstonia solanacearum
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, Hebbal, Bangalore 560 024, IN
1 National Bureau of Agriculturally Important Insects, Hebbal, Bangalore 560 024, IN
Source
Journal of Biological Control, Vol 27, No 3 (2013), Pagination: 217–220Abstract
Bacillus megaterium strain NBAII 63 was identified as a potential bacterial antagonist against brinjal bacterial wilt pathogen, Ralstonia solanacearum. It was tested for its ability to induce defense related enzymes viz., peroxidase (PO), polyphenoloxidase (PPO) and total phenols against R. solanacearum in brinjal plants. Brinjal plants treated with B. megaterium challenge inoculated with R. solanacearum showed higher levels of defense related enzymes and phenols compared to antagonist alone, pathogen alone and untreated plants. B. megaterium strain NBAII 63 showed the higher activities of total phenols (173 μg g-1 of tissue compared to control 121), PO (2.75 change in OD min-1g-1 of tissue compared to control 0.75) and PPO activity (0.91 change in OD min-1g-1 compared to control 0.13) in brinjal plants treated with R. solanacearum. The present study clearly indicated that B. megaterium strain NBAII 63 has the ability to induce the defense related enzymes in the brinjal plants against R. solanacearum.Keywords
Induced Defense, Brinjal, Bacillus megaterium, Bacterial Wilt, Ralstonia solanacearum.References
- Anand T, Chandrasekaran A, Kuttalam S, Senthilraja G, Samiyappan R. 2010. Integrated control of fruit rot and powdery mildew of chilli using the biocontrol agent Pseudomonas fluorescens and a chemical fungicide. Biol Control 52: 1–7.
- Choudhary DK, Johri BN. 2009. Interactions of Bacillus spp. and plants – with special reference to induced systemic resistance (ISR). Microbiol Res. 164: 49– 513.
- De Vecchi L, Matta A. 1989. An ultrastructural and cytochemical study of peroxidases, polyphenoloxidases, and phenols in xylem of tomato plant infected with Fusarium oxysporum f. sp. lycopersici or Fusarium oxysporum f. sp. melonis. Caryologia 42: 103–114.
- Gupta VP, Bochow H, Dolej S, Fischer. 2000. Plant growthpromoting Bacillus subtilis strain as potential inducer of systemic resistance in tomato against Fusarium wilt. Zeitschrifur fur Pflanzekrankheiten und Pflanzenschutz 107: 0340–8159.
- Grimault V, Schmit J, Prior. 1993. Some characteristics involved in bacteria resistance in tomato, Pp: 112– 119. In: Hartman, GL and Hayward, AC (eds.), Bacterial wilt. ACIAR proceedings No. 45: Australian centre for International Agriculture Research, Camera.
- Hammerschmidt R, Nuckles EM, Kuc J. 1982. Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiol Pl Path. 20: 73–82.
- Jayaraj J, Yi H, Liang GH, Muthukrishnan S, Velazhahan R. 2004. Foliar application of Bacillus subtilis AUBS1 reduces sheath blight and triggers defense mechanisms in rice. J Pl Dis Prot. 111: 115–125.
- Kloepper JW, Ryu CM, Zhang S. 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopath. 94: 1259–66.
- Malik CP, Singh M B. 1980. Plant enzymology and Histo Enzymology, Kalyani Publishers, New Delhi, pp. 53.
- Mayer AM, Harel E, Shaul RB.1965. Assay of catechol oxidase, a critical comparison of methods. Phytochemistry 5: 783–789.
- Nagorska K, Bikowski M, Obuchowskji M. 2007. Multicellular behaviour and production of a wide variety of toxic substances support usage of Bacillus subtilis as a powerful biocontrol agent. Acta Biochemica Polonica 54: 495–508.
- Nakkeeran S, Kavitha K, Chandrasekar G, Renukadevi P, Fernando WGD. 2006. Induction of plant defense compounds by Pseudomonas chlororaphis PA23 and Bacillus subtilis BSCBE4 in controlling damping-off of hot pepper caused by Pythium aphanidermatum. Biocontrol Sci Tech. 16: 403–416.
- Nicholson RL, Hammerschmidt R.1992. Phenolic compound and their role in disease resistance. Annu Rev Phytopath. 30: 369–389.
- Ramanujam B, Basha H, Vinaya H, Chowdappa P, Rangeshwaran R. 2012. Induction of defense related enzymes and phenols in chilli plants by Bacillus subtilis against anthracnose pathogen, Colletotrichum capsici. Phytopath. 65(4): 382–385.
- Sivakumar G, Rangeshwaran R, Sriram S. 2011. Screening and identification of potential spp. for the management of bacterial wilt of brinjal (egg plant). J Biol Control 25: 229–235.
- Sivakumar G, Rangeshwaran R. 2013. Evaluation of Bacillus megaterium strain NBAII 63 against bacterial wilt of brinjal (Solanum melongena). J Myco Pl Path. 43(1): 95–98.
- Thilagavathi R, Saravanakumar D, Ragupathi N, Samiyappan R. 2007. A combination of biocontrol agents improves the management of dry ischolar_main rot (Macrophomina phaseolina) in greengram. Phytopath Mediterranean 46: 157–167.
- Bioefficacy of Peat Formulation of Bacterial Antagonists on Growth Promotion and Disease Suppression in Cardamom (Elettaria cardamomum Maton)
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
2 Cardamom Research Station, Pampadumpara 685 566, Idukki, Kerala, IN
1 National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
2 Cardamom Research Station, Pampadumpara 685 566, Idukki, Kerala, IN
Source
Journal of Biological Control, Vol 26, No 3 (2012), Pagination: 255–259Abstract
Among the 90 rhizobacterial isolates screened against capsule rot pathogen of cardamom Phytophthora meadii Mc Rae two bacterial strains viz., Pseudomonas fluorescens Pf 51 and Bacillus subtilis Bs were found highly inhibitory. Strain Pf 51 exhibited highest inhibition (40.2%) against P. meadii. Similarly B.subtilis strain Bs also exhibited highest inhibition (39.7%) against P. meadii. P. fluorescens strain Pf51 was found compatible with strain B. subtilis Bs. Application of antagonists both Pf 51 and Bs in combination with rhizome bacterization and soil application resulted in 60% reduction of capsule infection over control as compared to single methods such as rhizome bacterization (53%) and soil application (46%). Application of copper oxy chloride resulted in 73% reduction of capsule infection. Maximum height (169.7cm) and number of tillers (36.3) were recorded due to the application of mixture of both the strains through rhizome bacterization and soil application.Keywords
Cardamom, Capsule Rot, Bacterial Antagonists, Peat Formulation.References
- Bharathi R, Vivekananthan R, Harish S, Ramanathan A,Samiyappan R. 2004. Rhizobacteria-based bioformulationsfor the management of fruit rot infectionin chillies. Crop Prot. 23: 835–843.
- Bloemberg GV, Lugtenberg BJJ. 2001. Molecular basisof plant growth promotion and biocontrol byrhizobacteria. Current Opinion in Plant Biol. 4:343–350.
- Bochow H, Dolej S.1999. Mechanisms of toleranceinduction in plants by ischolar_main colonizing Bacillussubtilis isolates, pp. 411–416 In: Lyr H (Eds.). Proceedings 12th International ReinhardsbrunnSymposium on Modern Fungicides and AntifungalCompounds, Thuringia, Germany, InterceptPublications, UK.
- Pal KK, Dey R, Bhat DM, Chauhan, SM. 2000. Enhancement of groundnut growth yield by plantgrowth promoting rhizobacteria. Int Arachis NewsLett. 19: 51–53.
- Rajan PP, Sarma YR, Anandaraj M. 2002. Management offoot rot disease of black pepper with Trichodermaspp. Indian Phytopathol. 55: 34–38.
- Rangeshwaran R, Prasad RD. 2000. Isolation and screeningof rhizobacteria for control of chickpea diseases. J Biol Control, 14: 9–15.
- Raupach GS, Kloepper JW.1998. Mixtures of plant growthpromoting rhizobacteria enhanced biological controlof multiple cucumber pathogens. Phytopathol. 88:1158–1164.
- Salaheddin K, Valluvaparidasan V, Ladhalakshmi D,Velazhahan R. 2010. Management of bacterial blightof cotton using a mixture of Pseudomonas fluorescensand Bacillus subtilis. Plant Prot Sci. 46: 41–50.
- Sivakumar G, Sharma, RC. 2007. Management of bandedleaf and sheath blight of maize using Pseudomonasfluorescens, SAARC J Agri. 5: 79–85.
- Sivakumar G, Rangeshwaran R, Sriram S. 2011. Screeningand identification of potential Bacillus spp. formanagement of bacterial wilt of brinjal. J Biol Control25: 229–235.
- Thomas J, Vijayan AK. 2003. PGPR induced growthpromotion biocontrol activity in small cardamom. In: Reddy MS, Anandaraj M, Santhosh J Eapen,Sarma YR, Kumar A. (Eds.). In 6th InternationalWorkshop on Plant Growth Promoting Bacteria.Abstracts and Short Papers, 5–10 October 2003,Calicut (p. 44). Indian Society for spices, Calicut.
- Tsao PH, Guy SO.1977. Inhibition of Mortierella andPythium in a Phytophthora isolation mediumcontaining hymexazole, Phytopathol. 67: 796–801.
- Screening and in Vitro Evaluation of Native Pseudomonas Spp., against Nematode Pathogens and Soil Borne Fungal Pathogens
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Authors
Affiliations
1 Central Plantation Crops Research Institute, Kasargod 671 124, Kerala, IN
2 National Bureau of Agriculturally Important Insects (NBAII), Bangalore 560 024, IN
1 Central Plantation Crops Research Institute, Kasargod 671 124, Kerala, IN
2 National Bureau of Agriculturally Important Insects (NBAII), Bangalore 560 024, IN
Source
Journal of Biological Control, Vol 27, No 4 (2013), Pagination: 305–311Abstract
The objective of this study was to assess the efficacy of native Pseudomonas spp., against ischolar_main-knot nematode, Meloidogyne incognita and other soil borne fungal pathogens such as Fusarium oxysporum f. sp. Lycopersici and Sclerotium rolfsii. The eggs and second stage juveniles (J2) of M. incognita were exposed to each isolates of Pseudomonas spp., by diluting the standard culture filtrate to fifty percent and to undiluted culture filtrate (100%). Four isolates of Pseudomonas spp. (CRS3, CRS6, and CRS8 and CRS10) significantly induced inhibition of egg hatching and mortality of M. incognita juveniles. The per cent mortality was proportional to the concentration of culture filtrate and the duration of exposure period. The highest percentage of inhibition of egg hatching was recorded for CRS3 while mortality of second stage juveniles was found in the case of CRS10 in undiluted culture filtrate. The CRS6 caused 48% inhibition of Sclerotium rolfsii while CRS8 caused 58% inhibition of Fusarium oxysporum f. sp. lycopersici. Since meloidogyne infection can predispose plants to plant pathogens, these isolates show promise for management of nematode and disease complex of vegetable crops.Keywords
Culture Filtrate, Egg Hatching, Meloidogyne Incognita, Pseudomonas Spp., Root-knot Nematode and Soil Borne Fungi.References
- Anonymous 1957. Manual of microbiological methods. Society of American Bacteriologists, McGraw Hill Book Company, Inc., New York, 315 pp.
- Ashraf MS, Khan TA. 2010. Integrated approach for the management of Meloidogyne javanica on eggplant using oil cakes and bio-control agents. Arch. Phytopathology. Pl Prot. 43: 609–614.
- Becker JO, Zavaleta-Mejia, Colbert SF, Schroth MN, Weinhold AR, Hancock JG, Van Gundy SD. 1988.
- Effects of rhizobacteria on ischolar_main-knot nematodes and gall formation. Phytopathol 78: 1466–1469.
- Buchanan RE, Gibbons NE. 1974. Bergey’s Manual of Determinative Bacteriology. Baltimore, Williams & Wilkins, 8th edition, pp. 13–45.
- Carter CC. 1985. Literature search: host range of Meloidogyne hapla. Internatl Nematol Network Newslett. 2: 16–24.
- Cayrol JC, Djian C, Pijarowski I. 1989. Studies on the nematicidal properties of the culture filtrate of the nematophagous fungus Paecilomyces lilacinus. Revue de Nematologie 12: 331–336.
- Dawar S, Wahab S, Tariq M, Zaki MJ. 2010. Applications of Bacillus species in the control of ischolar_main-rot disease of crop plants. Arch Phytopathol Pl Prot. 43 (4): 412–418.
- Gokte N, Swarup G. 1988. On the potential of some bacterial biocides against ischolar_main-knot and cyst nematodes. Ind J Nematol. 18: 152–153.
- Goswami BK, Bhattacharya Chaitali, Singh Neetu, Paul MP Reject.2012. An IPM package for the management of soil borne fungal and ischolar_main-knot nematode diseases on tomato and okra. Pesticide Res J. 24(1): 91–95.
- Hanna AL, Raid FW, Tawfik AE. 1999. Efficacy of antagonistic rhizobacteria on the control of ischolar_main-knot nematode, Meloidogyne incognita in tomato plants. Egyptian J Agri Res. 77: 1467–1476.
- Hartman KM, Sasser JN. 1985. Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. pp. 69–77. In: Barker KR, Carter, CC, Sasser JN (ed.). An Advanced Treatise on Meloidogyne. Vol II, methodology, North Carolina State University Graphics, Raleigh, NC, USA. Hussey RS, Barker KR. 1973. A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Pl Dis Rep. 61: 328–331.
- Ioannis K, Vagelas, Baerbark Pembroke, Simon R, Gowen and Keith G. Davies. 2007. The control of ischolar_main-knot nematodes (Meloidogyne spp.) by Pseudomonas oryzihabitans and its immunological detection on tomato ischolar_mains. Nematol. 9 (3): 363–370. Johnsson L, Hökeberg M, Gerhardson B. 1998. Performance of the Pseudomonas chlororaphis, biocontrol agent MA 342 against seed-borne diseases in field experiments. European J Pl Pathol. 104: 701–711.
- Khan Z, Park SD, Shin SY, Bae SG, Yeon IK, Seo YJ. 2005. Management of Meloidogyne incognita on tomato by ischolar_main dip treatment in culture filtrate of the blue green alga, Microcoleus vaginatus. Biores Technol. 96: 1338–1341.
- Kienwnick S, Sikora RA. 2006. Biological control of the ischolar_main-knot nematode, Meloidogyne incognita by Paecilomyces lilacinus strain 251. Biol Cont. 38: 179– 187.
- King EO, Ward MK, Raney DE. 1954. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 11: 441–449.
- Knight KWL, Barber CJ, Page GD. 1997. Plant-parasitic nematodes of New Zealand recorded by host association. J Nematol. 29: 640–656.
- Kuruganti K. 2005. Effects of pesticide exposure on developmental task performance in Indian children, Youth and Environ. 15 (1): 83–114.
- Li bin, Xie Guan-lin, Soad A, Coosemans J. 2005. Suppression of Meloidogyne javanica by antagonistic and plant growth promoting rhizobacteria. J Zhejjang. 496–501.
- Mohamed Hashem, Kamal Abo-Elyousr A. 2011. Management of ischolar_main-knot, Meloidogyne incognita on tomato with combinations of different bio-control organisms. Crop Prot. 30: 1–8.
- Mujeebur Rahman Khan, Shaharana Majid, Fayaz A, Mohidin, Nibilah Khan. 2011. A new bioprocess to produce low cost powder formulations of bio-control bacteria and fungi to control fusarial wilt and ischolar_mainknot nematodes of pulses. Biol Cont. 59: 130–140.
- Nash SN, Snyder WC. 1962. Quantitative estimation of plate counts of propagules of the bean ischolar_main rot Fusarium in field soils. Phytopathol. 52: 567–572.
- Oostendorp M, Sikora RA. 1990. In vitro inter-relationships between rhizosphere bacteria and Heterodera schachtii. Rev de Nematol. 13: 269–274.
- Reddy Parvatha P.1986. Chemical control of plant parasitic nematodes infecting citrus and grapevine. Ind J Hort 43: 303–305.
- Regina MDGC, DeSouza IS, Belarmino LC. 1998. Nematicidal activity of Bacillus spp. strains on juveniles of Meloidogyne javanica. Nemat Brasiliera 22: 12–21.
- Sasser JN. 1979. Economic importance of Meliodogyne in tropical countries, pp. 359–374. In: Lamberti, F., Tylor, C. E. (Eds.) Root-knot nematodes (Meloidogyne species) Systematics, Biology & Control. Academics Press. London.
- Shankra T, Pavaraj M, Umamaheswari Prabhu D, Baskaran S. 2011. Effect of Pseudomonas aeruginosa on the ischolar_main-knot nematode, Meloidogyne incognita infecting tomato, Lycopersicum esculentum. A J Ent. 4 (3): 114–117.
- Sharma SB, Rupela OP, Ansari MA, Gopalkrishnan S. 1998. Suppression of Meloidogyne javanica egg hatch by on isolate of Pseudomonas striata. In: III International Symposium of Afro-Asian Society of Nematologists, 16-19 April 1998, Coimbatore, India.
- Siddiqui ZA, Ehteshamul-Haque S, Shukat SS. 2001. Use of rhizobacteria in the control of ischolar_main rot- ischolar_main-knot disease complex of mungbean. J Phytopathol. 149: 337–346.
- Siddiqui I, Shukat S. 2003. Suppression of ischolar_main diseases by Pseudomonas fluorescenes CHAO in tomato: Importance of bacterial secondary metabolities 2, 4-diacetylphloroglucinol. Soil Biol Biochem. 35: 1 615 – 1623.
- Siddiqui ZA, Mahmood I. 1999. Role of bacteria in the management of plant parasitic nematodes: a review. Bio-resource Tech. 69: 167–179.
- Vagelas IK. 2002. Efficacy of Pseudomonas oryzihabitans as bio-control agent of ischolar_main pathogens. Ph.D. Thesis, Department of Agriculture, University of Reading, UK.
- Vagelas IK, Gravanis FT, Gowen SR. 2003. Control of Fusarium oxysporum and Meloidogyne spp. with Pseudomonas oryzihabitans. In: Proceedings of the BCPC International Congress – Crop Science and Technology. Vol. 1. Glasgow, UK., 419–424.
- Characterization of Abiotic Stress Tolerant Pseudomonas Spp. Occurring in Indian Soils
Abstract Views :375 |
PDF Views:128
Authors
K. Ashwitha
1,
R. Rangeshwaran
1,
N. V. Vajid
2,
G. Sivakumar
1,
S. K. Jalali
1,
K. Rajalaksmi
3,
H. Manjunath
4
Affiliations
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
2 Department of Biology, University of Hail, Jeddah, SA
3 Department of Plant Pathology, University of Agricultural Sciences, Dharwad 580 005, Karnataka, IN
4 Department of Plant Pathology, GKVK, UAS Bangalore 560 065, IN
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
2 Department of Biology, University of Hail, Jeddah, SA
3 Department of Plant Pathology, University of Agricultural Sciences, Dharwad 580 005, Karnataka, IN
4 Department of Plant Pathology, GKVK, UAS Bangalore 560 065, IN
Source
Journal of Biological Control, Vol 27, No 4 (2013), Pagination: 319–328Abstract
Abiotic stress tolerance of 230 Pseudomonas spp. Occurring in Indian soils was evaluated for tolerance to temperature, salinity and moisture stresses. Forty seven Pseudomonas spp. Were characterized as abiotic stress tolerant and were identified as P. aeruginosa (24), P. putida (14), P. plecoglossicida (4), P. mosselli (1), Pseudomonas sp. (1) and P. fluorescens (3). The temperature and salinity tolerance of these bacteria was 45°C and 1 M NaCl respectively. Most isolates (44 out of 47) produced indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and 37 of them showed phosphatase activity. 2, 4-diacetyl phloroglucinol (DAPG) gene was detected in 10 isolates and pyoluteorin gene was detected in 4 isolates. Under water stress, seed treatment with P. putida (NBAII-RPF9) and P. fluorescens (PFDWD) showed its potential as plant growth promoter. The studies also indicated that stress tolerant Pseudomonas spp. may be used as plant protection agents in abiotically stressed soils.Keywords
Pseudomonas, Abiotic Stress, Tolerance, DAPG, Pyoluteorin.References
- Ali ZSK, Sandhya V, Minakshi G, Kishore N, Rao LV, Venkateswarlu B. 2009. Pseudomonas sp. strain AKMP6 enhances tolerance of sorghum seedlings to elevated temperatures. Biol Fert Soils 46: 45–55.
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402.
- Bric JM, Bostock RM, Silverstone SE. 1991. Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl Environ Microbiol. 57: 535–538.
- Delauney AJ, Verma DPS. 1993. Proline biosynthesis and osmoregulation in plants. Pl J. 4: 215–223.
- Dimkpa C, Weinand T, Asch F. 2009. Plant-rhizobacteria interactions alleviate abiotic stress conditions. Pl Cell Environ. 32: 1682–1694.
- Djedidi S, Yokoyama T, Ohkama-Ohtsu N, Risal CP, Abdelly C, Sekimoto H. 2011. Stress tolerance and symbiotic and phylogenic features of ischolar_main nodule bacteria associated with medicago species in different bioclimatic regions of tunisia. Microbes Environ. 26: 36–45.
- Egamberdieva D. 2011. Survival of Pseudomonas extremorientalalis TSAU20 and P. chlororaphis TSAU13 in the rhizosphere of common bean (Phaseolus vulgaris) under saline conditions. Pl Soil Environ. 57: 122–127.
- Egamberdieva D, Kamilova F, Validov S, Gafurova L, Kucharova Z, Lugtenberg B. 2008. High incidence of plant growth-stimulating bacteria associated with the rhizosphere of wheat grown on salinated soil in Uzbekistan. Environ Microbiol. 10: 1–9.
- Gordon SA, Weber RP. 1951. Colorimetric estimation of indole acetic acid. Pl Physiol. 26: 192–195.
- Hammer PE, Hill DD, Lam ST, Van Pée KH, Ligon JM. 1997. Four genes from Pseudomonas fluorescens that encode the biosynthesis of pyoluteorin. Appl Environ Microbiol. 63: 2147.
- Keyvan S. 2010. The effects of drought stress on yield, relative water content, proline, soluble carbohydrates and chlorophyll of bread wheat cultivars. J Anim Pl Sci. 8: 1051–1060.
- Knapp S, Schultz MJ, Van der Poll T. 2005. Pneumonia models and innate immunity to respiratory bacterial pathogens. Shock 24: 12–18.
- Mahmood M, Rahman ZA, Saud HM, Shamsuddin ZH, Subramaniam S. 2010. Influence of rhizobacterial and agrobacterial inoculation on selected physiological and biochemical changes of banana cultivar, berangan (AAA) plantlets. J Agric Sci. 2: 115–137.
- Mayak S, Tirosh T, Glick B. 2004. Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Pl Physiol Biochem. 42: 565–572.
- Naik PR, Sahoo N, Goswami D, Ayyadurai N, Sakthivel N. 2008. Genetic and functional diversity among fluorescent pseudomonads isolated from the rhizosphere of banana. Microbiol Ecol. 56: 492–504.
- Principe A, Alvarez F, Castro MG, Zachi L, Fischer SE, Mori GB, Jofr E. 2008. Biocontrol and PGPR Features in native strains isolated from saline soils of argentina. Curr Microbiol. 55: 314–322.
- Raajiijmakers JM, Weller DM, Thomashow LS. 1997. Frequency of antibiotic-producing Pseudomonas spp. in natural environments. Appl Environ Microbiol. 63: 881–887.
- Rangarajan S, Saleena LM, Vasudevan P, Nair S. 2003. Biological suppression of rice diseases by Pseudomonas spp. under saline soil conditions. Pl Soil 251: 73–82.
- Ritchie SW, Nguyan HT, Holaday AS. 1990. Leaf Water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Sci. 30: 105–111.
- Ruiz-Díez B, Fajardo S, Puertas-Mejía MA, de Felipe MR, Fernández-Pascual M. 2009. Stress tolerance, genetic analysis and symbiotic properties of ischolar_main-nodulating bacteria isolated from Mediterranean leguminous shrubs in Central Spain. Arch Microbiol. 191: 35–46.
- Saleem M, Arshad M, Hussain L, Bhatti AS. 2007. Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture. J Ind Microbiol Biotech. 34: 635–648.
- Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. Sandhya V, Ali ZSk, Minakshi G, Reddy G, Venkateswarlu B. 2010. Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress. Pl Gr Reg. 62: 21–30.
- Saravanakumar D, Kavino M, Raguchander T, Subbian P, Samiyappan R. 2011. Plant growth promoting bacteria enhance water stress resistance in green gram plants. Acta Physiol Plant. 33: 203–209.
- Shinde BM, Limaye AS, Deore GB, Laware SL. 2010. Physiological response of groundnut (Arachis hypogaea L.) varieties to drought stress. Asian J Exp Biol Sci. 65–68.
- Trabelsi D, Mengoni A, Mohammed Elarbi Aouani ME, Mhamdi R, Bazzicalupo M. 2009. Genetic diversity and salt tolerance of bacterial communities from two Tunisian soils. Ann Microbiol. 59: 25–32.
- Vyas P, Rahi P, Gulati A. 2009. Stress tolerance and genetic variability of phosphate-solubilizing fluorescent pseudomonas from the cold deserts of the trans-himalayas. Microbial Ecol. 58: 425–434.
- Weisburg WG, Barns SM, Lane DJ. 1991. 16S ribosomal DNA amplification for phylogenetic study. J Bact. 173: 697–703.
- Weller DM, Landa BB, Mavrodi OV, Schroeder KL, De La Fuente L, Bankhead SB, Molar RA, Bonsall RF, Mavrodi DV, Thomashow LS. 2007. Role of 2, 4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. in the defense of plant ischolar_mains. Pl Biol. 9: 4–20.
- Yao Y, Wu Z, Zheng Y, Kaleem I, Li C. 2010. Growth promotion and protection against salt stress by Pseudomonas putida Rs-198 on cotton. Eur J Soil Biol. 46: 49–54.
- Yun-xiu J, Xiao-dong H. 2007. Effects of plant growthpromoting rhizobacteria on the seedling growth of oat and annual ryegrass under salt stress. In: Proceedings of the International Conference on Agricultural Engineering; St. Plum Blossom Press Pvt Ltd. (Australia).
- Identification of Endophytic Bacteria in Chickpea (Cicer arietinum L.) and their Effect on Plant Growth
Abstract Views :284 |
PDF Views:138
Authors
Affiliations
1 Biological Control, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore - 560024, Karnataka, IN
2 Department of Agricultural Microbiology, University of Agricultural Sciences, Bangalore - 560065, Karnataka, IN
1 Biological Control, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore - 560024, Karnataka, IN
2 Department of Agricultural Microbiology, University of Agricultural Sciences, Bangalore - 560065, Karnataka, IN
Source
Journal of Biological Control, Vol 22, No 1 (2008), Pagination: 13-23Abstract
Five endophytic bacteria were isolated from healthy chickpea (Cicer arietinum L.) plants by surface-disinfestation method and identified based on morphological, physiological and biochemical tests. Erwinia herbicola and Enterobacter agglomerans, which had nitrate-solubilizing ability, were isolated from the ischolar_main endosphere and Bacillus megaterium, B. circulans and one unidentified species of Bacillus were isolated from the leaf and stem tissues. None of the isolates had phosphate-solubilizing ability. All the isolates tolerated a wide range of pH and exhibited growth from pH 5 to 9. B. circulans and E. agglomerans tolerated an alkaline pH of 11, which is unusual. Higher growth promotion was noticed in chickpea plants treated with B. megaterium, E. agglomerans and Bacillus sp. and the plants treated with endophytes survived well in the presence of the wilt pathogen, Rhizoctonia solani. Seedlings from seeds treated with bacteria showed an increase in phenol content up to sixth day after Inoculation. The maximum phenol content (483.33μg g−1) was noticed in B. megaterium treated and the lowest (246.67μg g−1) was in control. Endophytic bacteria from healthy plant tissue could play a useful role In plant protection.Keywords
Chickpea, Endophytic Bacteria, Growth Promotion, Identification.- Resistance and Susceptibility Pattern of Chickpea (Cicer arietinum L) Endophytic Bacteria to Antibiotics
Abstract Views :197 |
PDF Views:137
Authors
Affiliations
1 Biological Control (ICAR), H. A. Farm Post, Hebbal, Bellary Road, Bangalore, 560 024, Karnataka, IN
2 Department of Agricultural Microbiology, University of Agricultural Sciences, Bangalore, Karnataka, IN
1 Biological Control (ICAR), H. A. Farm Post, Hebbal, Bellary Road, Bangalore, 560 024, Karnataka, IN
2 Department of Agricultural Microbiology, University of Agricultural Sciences, Bangalore, Karnataka, IN
Source
Journal of Biological Control, Vol 22, No 2 (2008), Pagination: 393-403Abstract
Five chickpea (Cicer arietinum) endophytic bacteria, identified as Erwinia herbicola, Enterobacter agglomeraus. Bacillus megaterium and Bacillus sp. and Bacillus circulans were tested for intrinsic antibiotic resistance in order to see if endophytes showed variation in resistance to antibiotics. The resistance pattern was compared with two rhizospheric bacteria viz. Psendomonas fluoresceus and B. subtilis in order to see if the susceptibility of endophytes differed with that of bacteria isolated from rhizosphere. The endophytes seemed to be less resistant to antibiotics. B. circulaus was susceptible to all antibiotics tested except amoxycillin (10μg). However B. megaterium and Bacillus sp, and E. agglomeraus showed some resistance, P. fluorescens and B. subtilis showed resistance to a wide range of antibiotics indicating that they could be better competitors in the rhizosphere. Preliminary screening was done to monitor B. megaterium and Bacillus sp. by using the observed antibiotic resistance. Out of the 25 ischolar_main/stem/leaf tissues tested, 10 tested positive for the presence of B. megaterium and 11 for Bacillus sp. However, they coutd not be reisolated from the stem tissue, 3 and 2 of the leaf samples showed presence of B. megaterium and Bacillus sp., respectively.Keywords
Antibiotics, Endophytic Bacteria, Resistance Pattern.- Isolation of Endophytic Bacteria for Biological Control of Wilt Pathogens
Abstract Views :281 |
PDF Views:161
Authors
Affiliations
1 Project Directorate of Biological Control (ICAR), Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
2 Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwa Vidhyalaya, Jabalpur 482 004, M. P., IN
1 Project Directorate of Biological Control (ICAR), Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
2 Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwa Vidhyalaya, Jabalpur 482 004, M. P., IN
Source
Journal of Biological Control, Vol 16, No 2 (2002), Pagination: 125-134Abstract
Twentyfive endophytic bacteria were isolated from internal tissues of ischolar_main and stem portions of chickpea, sunflower, niger, chilli and capsicum plants. The endophytes were screened in dual culture on Potato Dextrose Agar (PDA) and Tryptic Soya Agar (TSA) against Fusarium oxysporum f. sp. ciceri, Fusarium udum, Rhizoctonia solani and Sclerotium rolfsii. Ten isolates exhibited inhibition of the pathogens. Maximum percent inhibition (37.93) of F. oxysporum f. sp. ciceri was obtained on PDA with B. subtilis (PDBCEN 3). On TSA percent inhibition was maximum (52.21) with isolate PDBCEN-7. Testing against F. udum in dual culture test revealed that Pseudomonas sp. (PDBCEN 8) showed maximum (40.45%) inhibition on PDA. Pseudomonas sp. (PDBCEN-2) was highly effective on TSA and showed maximum (56.9%) inhibition zone. Against R. solani, maximum inhibition (44.96%) was recorded with endophyte PDBCEN 7. On TSA all the ten endophytic bacteria were effective in restricting the growth of test fungus. Percent inhibition of S. rolfsii was maximum (40.93%) with Pseudomonas sp. (PDBCEN 6) on PDA. On TSA percent inhibition was maximum (46.73%) with P. fluorescens (PDBCEN 1). The endophytic isolates were able to promote better growth of chickpea but the vigour index varied between the isolates. We could not correlate high pathogen inhibition under in vitro with high vigour index.Keywords
Biological Control, Endophytic Bacteria, Wilt Pathogens.- Bioefficacy and Shelf Life of Conidial and Chlamydospore Formulations of Trichoderma harzianum Rifai
Abstract Views :303 |
PDF Views:279
Authors
Affiliations
1 Project Directorate of Biological Control (ICAR), P. B. No. 2491, H. A. Farm Post, Hebbal, Bangalore 560 024, Karnataka, IN
1 Project Directorate of Biological Control (ICAR), P. B. No. 2491, H. A. Farm Post, Hebbal, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 16, No 2 (2002), Pagination: 145-148Abstract
The conidia and chlamydospores of Trichoderma harzianum produced by solid state and liquid fermentation, respectively, were formulated as powder and tested for their comparative bioefficacy and shelf life. Both the propagules did not differ significantly in reducing ischolar_main rot incidence in chickpea caused by Rhizoctonia solani. Conidial formulation retained optimum amount of viable propagules (>106 cfu/g) even after 180 days of storage at room temperature but in chlamydospore formulation, viable propagules reduced to less than 106 by 150 days.Keywords
Bioefficacy, Chlamydospores, Conidia, Formulation, Shelf Life, Trichoderma harzianum.- A Rapid in vivo Bioassay Method for Testing and Selection of Fungal Antagonists of Plant Pathogens
Abstract Views :223 |
PDF Views:122
Authors
Affiliations
1 Crop Research Station, Narendra Deva University of Agriculture and Technology, Bahraich 271 801, U. P., IN
2 Project Directorate of Biological Control (ICAR), P. B. No. 2491, H. A. Farm Post, Hebbal, Bangalore 560 024, Karnataka, IN
3 Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwa Vidhyalaya, Jabalpur 482 004, M. P., IN
1 Crop Research Station, Narendra Deva University of Agriculture and Technology, Bahraich 271 801, U. P., IN
2 Project Directorate of Biological Control (ICAR), P. B. No. 2491, H. A. Farm Post, Hebbal, Bangalore 560 024, Karnataka, IN
3 Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwa Vidhyalaya, Jabalpur 482 004, M. P., IN
Source
Journal of Biological Control, Vol 16, No 2 (2002), Pagination: 173-176Abstract
Eight Trichoderma isolates were tested for their bioefficacy against seed, ischolar_main and seedling rot incited by Rhizoctonia solani by adopting an in vivo test method (blotter test). Vigor index ranging from 169.5 to 2239.4 and disease incidence ranging between 10 to 86 percent were recorded in various treatments. All bioagents were graded based on disease grading key proposed for their rating. The clear differentiation of efficacy of various Trichoderma species against R. solani obtained with the in vivo bioefficacy test method adopted (blotter test) in the present study shows suitability of this method for routine screening of fungal biocontrol agents against seed and soil borne plant pathogens.Keywords
Fungal Antagonists, in vivo Bioassay, Plant Pathogens.- Field Evaluation of Two Bacterial Antagonists, Pseudomonas putida (PDBCAB 19) and P. fluorescens (PDBCAB 2) against Wilt and Root-Rot of Chickpea
Abstract Views :185 |
PDF Views:140
Authors
Affiliations
1 Project Directorate of Biological Control (lCAR) Post Bag No.2491, H. A. Farm Post, Bellary Road Bangalore 560024, Karnataka, IN
1 Project Directorate of Biological Control (lCAR) Post Bag No.2491, H. A. Farm Post, Bellary Road Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 15, No 2 (2001), Pagination: 165-170Abstract
Talc based formulations of two antagonistic bacteria viz., Pseudomonas putida (PDBCAB 19) and P. fluorescens (PDBCAB 2) were evaluated against natural incidences of wilt and wet ischolar_main-rot of chickpea. Observations on rhizoclonia ischolar_main-rot incidence indicated that the disease was prevalent up to 60 days of plant growth whereas fusarial wilt was observed from 60 days. At 30th day. highest ischolar_main-rot incidence (10.8%) was observed in pathogen control plots and minimum was in fungicide treated plots (3.1 %). P. fluorescens (PDBCAB 2) treated plots also exhibited low ischolar_main-rot (4.4%). However, at day 60 lowest ischolar_main-rot incidence (5%) was recorded in P. fluorescens (PDBCAB 2) treated plots and highest ischolar_main-rot incidence (13.9%) was observed in pathogen control. Low ischolar_main-rot incidence (5.8%) was also noticed in P. putida (PDBCAB 19) treated plots. Fusarium wilt was lowest (3.3%) in P. putida (PDBCAB 19) treated and highest (13.3 %) in control plots at day 90. Combination of both P. fluorescens (PDBCAB 2) and P. putida (PDBCAB 19) exhibited suppression of ischolar_main-rot and wilt incidences by 5.8 and 7.5 per cent, respectively. Highest plant stand and seed yield was observed in P. fluorescens (PDBCAB 2) treated plots. There were no significant differences in the shoot and ischolar_main lengths among bioagent treatments, however, vigour index was highest in plots treated with a combination of P. fluorescens (PDBCAB 2) and P. putida (PDBCAB 19).Keywords
Antagonistic Bacteria, Chickpea, Root-Rot, Wilt.- Isolation and Evaluation of Rhizospheric Bacteria for Biological Control of Chickpea Wilt Pathogens
Abstract Views :210 |
PDF Views:122
Authors
Affiliations
1 Project Directorate of Biological Control (ICAR) Post Bag No. 2491, H. A. Farm Post, Bellary Road Hebbal, Bangalore 560024, Karnataka, IN
1 Project Directorate of Biological Control (ICAR) Post Bag No. 2491, H. A. Farm Post, Bellary Road Hebbal, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 14, No 1 (2000), Pagination: 9-15Abstract
Three hundred rhizospheric isolates of bacteria collected from different regions of Karnataka were screened for in vitro antagonism in dual culture on Tryptic Soya Agar (TSA) against five fungal pathogens viz., Botrytis cinerea, Macrophomina phaseolina, Sclerotium rolfsii, Rhizoctonia solani and Fusarium oxysporum f. sp. ciceris. Four isolates selected as potential antagonists were identified as Pseudomonas putida (PDBCAB 19), P. fluorescens (PDBCAB 2), P. fluorescens (PDBCAB 29) and P. fluorescens (PD BCAB 30) and their ischolar_main colonizing ability was tested. The total rhizosphere population was the highest (log cfu 6.4) for P.fluorescens (PDBCAB 29) after four days of germination. The rhizosphere population stabilized (log cfu 4.0 to 5.0) after eight days of germination. Three pathogens namely F. oxysporum f. sp. ciceris (wilt pathogen),R. solani and M.phaseolina (ischolar_main rot pathogens) were targeted by the four selected antagonists under greenhouse conditions. The maximum plant stand (100%) was observed with P.jluorescens (PDBCAB 29 and 30) treated pots for R. solani and M. phaseolina. P. putida (PDBCAB 19) and P. fluorescens (PDBCAB 30) were able to fully control F. oxysporum f. sp. ciceris. All the four antagonists promoted growth of chickpea. P. fluorescens isolates (PDBCAB 29 and 30) produced the maximum growth. Survival of P. fluorescens (PDBCAB 29) in a talc based formulation was monitored over a period of 90 days at room temperature.Keywords
Antagonism, Chickpea, Plant Growth Promotion, Rhizospheric Bacteria, Survival Talc, Wilt.- Biological Control of Meloidogyne incognita (kofoid and White 1919) Chitwood 1949 on Tomato by Verticillium chlamydosporium Goddard Cultured on Different Substrates
Abstract Views :207 |
Authors
Affiliations
1 Project Directorate of Biological Control Post Box No. 249 I, H. A. Farm Post, Bellary Road Hebbal, Bangalore 560024, Karnataka, IN
1 Project Directorate of Biological Control Post Box No. 249 I, H. A. Farm Post, Bellary Road Hebbal, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 14, No 1 (2000), Pagination: 39-43Abstract
The efficacy of Verticillium chlamydosporium Goddard cultured on different substrates such as sorghum grain, rice grain, broken wheat, maize grain and wheat bran, was tested against meloidogyne incognita (Kofoid and White 1919) Chitwood 1949 with tomato cv. Pusa Ruby under potted condition. All the substrates favoured the multiplication of V. chlamydosporium and enabled the fungus to suppress the galls, egg masses and nematode population. The degree of suppression of nematode by V. chlamydosporium varied with the substrates used and the per cent parasitization of egg masses and eggs of M. incognita ranged between 39 to 70 and 51 to 89, respectively. Verticillium chlamydosporium cultured on sorghum grain applied @10glplant as well as Sglplant was found to be superior to other substrates in terms of parasitization of egg masses (70 and 89.3 %) and eggs (63 and 69 %), respectively. A significant increase in growth of tomato plants was observed with V. chlamydosporium treated plants.Keywords
Biological Control, Meloidogyne incognita, Tomato, Verticillium chlamydosporium.Full Text
- Metarhizium majus and Metarhizium robertsii Show Enhanced Activity against the Coleopteran Pests Holotricha serrata and Oryctes rhinoceros
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PDF Views:141
Authors
Affiliations
1 ICAR-National Bureau of Agricultural Insects Resources, Bangalore – 560024, Karnataka, IN
2 Institute of Wood Science and Technology, Bangalore – 560 003, Karnataka, IN
3 Ashoka Trust for Research in Ecology and the Environment (ATREE), Bangalore – 560064, Karnataka, IN
1 ICAR-National Bureau of Agricultural Insects Resources, Bangalore – 560024, Karnataka, IN
2 Institute of Wood Science and Technology, Bangalore – 560 003, Karnataka, IN
3 Ashoka Trust for Research in Ecology and the Environment (ATREE), Bangalore – 560064, Karnataka, IN
Source
Journal of Biological Control, Vol 31, No 3 (2017), Pagination: 135-145Abstract
Studies were conducted to systematically isolate Metarhizium isolates from the insect cadavers and soils of South India. Morphological and PCR amplified sequences of 5.8S ITS regions and RNA polymerase II largest subunit (RPB1) gene regions were used to identify the isolates at species level. Eight Metarhizium isolates were isolated and initially identified by morphological and microscopic studies. Further identification was confirmed through 5.8SrRNA ITS and RPB1 analysis. They were identified as three isolates of M. robertsii J.F. Bisch., Rehner & Humber sp. nov. (ArMz3R, ArMz3S and ArMz6W), one isolate of M. majus (J.R. Johnst.) J.F. Bisch., Rehner & Humber (VjMz1W) and four isolates of M. anisopliae (WnMz1S, NlMz2S, BgMz2S and DhMz4R). Topical conidial suspensions (TCS) and powder based formulations (PBF) of the eight indigenous isolates of Metarhizium spp. that were isolated from insect cadavers and soils of South India were tested against coleopteran pests Holotricha serrata L. and Oryctes rhinoceros L. that cause serious damage to sugarcane and palm trees respectively. Against H. serrata TCS of M. robertsii (ArMz6W) was the most effective with an LC50 of 6.893×105 cfu/ml and caused 100% mortality against the 3rd instar larvae in 5 days; PBF elicited an LC50 of 7.502×105 cfu/ml with 96% mortality in 10 days. Against O. rhinoceros TCS (LC50 of 9.75×105 cfu/ml) of M. majus (VjMz1W) caused 90% mortality in 7 days and the PBF (LC50 of 9.57×105 cfu/ml) caused 86% mortality in 14 days. The results establish that M. robertsii is highly effective against H. serrata and against O. rhinoceros, M. majus was the most effective. The TCS formulations of these two strains can be readily deployed for field applications.Keywords
Holotricha serrata, Metarhizium spp., Oryctes rhinoceros, Powder Based Formulation (PBF), Topical Conidial Suspension (TCS).References
- Anitha V, Rogersb DJ, Wightmanc J, Wardd A. 2006. Distribution and abundance of white grubs (Coleoptera: Scarabaeidae) on groundnut in southern India. Crop Prot. 25: 732–740. Crossref.
- Bedford GO. 2013. Biology and Management of Palm dynastid Beetles: Recent Advances. Ann Rev Entomol. 58: 353–372. Crossref. PMid:23317044
- Bischoff SA, Rehner SA, Humber RA. 2009. A Multilocus pylogeny of Metarhizium anisopliae lineage. Mycologia 101: 512–530. Crossref. PMid:19623931
- Chandel RS, Mandeep Pathania1 KS, Verma BB, Sumit V, Vinod Kumar. 2015. The Ecology and Control of Potato, Whitegrubs of India. Potato Res. 58: 147–164. Crossref.
- Driver F, Milner RJ, Trueman WH. 2000. A taxonomic revision of Metarhizium based on a phylogenetic analysis of rDNA sequence data. Mycol Res. 104 (2): 134–150. Crossref.
- Easwaramoorthy S, Srikanth J, Santhalakshmi G, Geetha N. 2004. Laboratory and field studies on Beauveria brongniartii (Sacc.) Petch, against Holotrichia serrata F. (Coleoptera: Scarabaeidae) in sugarcane. Proceedings of the Annual Convention Sugar Technologists Association of India 66: 3–A19.
- Fleming WE. 1968. Biological control of the Japanese beetle. USDA-ARS Tech Bull No. 1383. 78.
- Hurpin B, Robert PH. 1972. Comparison of the activity of certain pathogens of the cockchafer Melolontha melolontha in plots of natural meadowland. J Invertebr Pathol. 19: 291–298. Crossref.
- Hu G, st. Leger RJ. 2002. Field studies using a recombinant mycoinsecticide (Metarhizium anisopliae) reveal that it is rhizosphere competent. Appl Environ Microbiol. 68: 6383–6387. Crossref. PMid:12450863 PMCid:PMC134390
- Metschnikoff VE . 1884. Ueber eine Sprosspilzkrank-heit der Daphnien. Beitrag zur Lehre über den Kampf der Phagocyten gegen Krankheit serreger. Virc Arcv. 96: 177–195.
- Nair CPR, Danielm M, Ponnamma KN. 1997. Integrated Pest Management in palms; Nambiar KKN, Nair MK (Eds.). Coconut Development Board, Kochi, India 30 pp.
- Nishi O, Iiyama K, Yasunaga-Aoki C, Shimizu S. 2011. Incongruence between EF-1α Phylogeny and Morphology of Metarhizium majus and Metarhizium guizhouense in Japan. Entomotech. 34: 19–23.
- Pradnya BM, Pandurang BM. 2014. Efficacy of newer molecules of insecticides against white grub in sugarcane. Asian J Bio Sci. 9:173–177. Crossref.
- Ramle M, Norman K, Ang, BN, Ramlah Ali SA, Basri MW. 2007. Application of powder formulation of Metarhizium anisopliae to control Oryctes rhinoceros in rotting oil palm residues under leguminous cover crops. J Oil Palm Res. 19: 318–330.
- Ramle M, Norman K, Mohd BW. 2009. Pathogenicity of granule formulations of Metarhizium anisopliae against the larvae of the oil palm rhinoceros beetle, Oryctes rhinoceros (L.). J Oil Palm Res. 2: 602–612.
- Sahayaraj K, Karthick S. 2008. Mass production of entomopathogenic fungi using agricultural products and by products. Afr J Biotechnol. 7(12): 1907–1910. Crossref.
- Shanmugam PS, Saravanan NA, Tamilselvan N. 2014. Assessment of biofungicides, Beauveria brongniartii and Metarhizium anisopliae against sugarcane white grub, Holotrichia serrata, Ind Jou Plant Prot. 42(4): 467–469.
- Srikanth J, Santhalakshmi G, Tamizharasi V. 2006. Viability and virulence of selected Beauveria brongniartii formulations against Holotrichia serrata. Sugar Tech. 8(2&3): 152–154. Crossref.
- Srikanth J, Santhalakshmi G, Nirmala R. 2011. An improved bioassay method for entomopathogenic fungi of sugarcane pests and its evaluation in studies of virulence in subcultures. Sugar Tech. 13(2): 156–165. Crossref.
- Tashiro H. 1973. Bionomics and control of ischolar_main feeding insect pests: grubs and billbugs. Bull Entomol Soc Am. 9: 92–94. Crossref.
- Thamarai Chelvi1 C, Thilagaraj RW, Nalini R. 2011. Field efficacy of formulations of microbial insecticide Metarhizium anisopliae (Hyphocreales: Clavicipitaceae) for the control of sugarcane white grub Holotrichia serrata F (Coleoptera :Scarabidae). J Biopest. 4(2): 186–189.4
- Veen KH, Ferron P. 1966. A selective medium for isolation of Beauveria tenella and of Metarrhizium anisopliae. J Invertebr Pathol. 8: 268–269. Crossref.
- Veeresh GK, Veerarajan, PV, Gubbaiah R, Vijaymohan N. 1977. Efficacy of certain soil insecticides against ischolar_main grubs (H. serrata F.) under irrigated and unirrigated conditions in tropical soils. Proceedings All India Symposiums on Soil Biology and Ecology, (2), pp. 23-31, 1977.
- Yamini Varma CK. 2013. Efficacy of ecofriendly management against Rhinoceros beetle grubs in coconut. J Biopest. 6(2): 101–103
- Diversity of cry Genes Occurring in the North East
Abstract Views :282 |
PDF Views:121
Authors
R. Rangeshwaran
1,
V. Velavan
2,
Satendra Kumar
2,
V. Apoorva
2,
K. M. Venugopala
2,
A. N. Shylesha
2,
G. Sivakumar
2
Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, H.A. Farm Post, Hebbal, Bellary Road, Bengaluru – 560024, Karnataka, IN
2 ICAR-National Research Centre for Banana, Thogaimalai Rd, Podavur, Thiruchirapalli – 620102, Tamil Nadu, IN
1 ICAR-National Bureau of Agricultural Insect Resources, H.A. Farm Post, Hebbal, Bellary Road, Bengaluru – 560024, Karnataka, IN
2 ICAR-National Research Centre for Banana, Thogaimalai Rd, Podavur, Thiruchirapalli – 620102, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 33, No 3 (2019), Pagination: 242-252Abstract
The search for new Bacillus thuringiensis (Bt) strains is a continuous process and researchers are now focusing on finding toxin proteins that are toxic to pests of insect orders that are not reported. In the present study soil and insect cadaver samples were collected from North East India comprising the states of Assam, Tripura and Mehhalaya and native Bt were isolated using standard protocols. At total of 30 Bt isolates were purified and characterized. Various types of crystal morphology were encountered that included bipyramidal, cuboidal, square, rhomboid, spherical and irregular. PCR analysis showed that diverse cry genes were expressed. The cry genes identified were Lepidoptera, Coleoptera and Diptera specific. Detected genes included cry1Ac, cry2A, cry4A, cry10A, cry16A, cry17A, cry19A, cry30Aa, cry44Aa, cry11A, cry4B, cry12A, cry8A and cry7A. Many of them were positive for Vip3A protein. The coleopteran specific Bt were evaluated against Sitophilus oryzae and Callosobruchus chinensis and NBAIR-AgBt6 was found to be toxic. The isolates are being further evaluated for use as biopesticides.Keywords
Bacillus thuringiensis, Bioassay, Cry Genes, Diversity, North East.References
- Aly AH Nariman. 2007. PCR Detection of cry genes in local Bacillus thuringiensis Isolates. Aust J Basic Appl Sci. 1(4): 461-466.
- Aronson AI. 1994. Bacillus thuringiensis and its use as biological insecticide. Plant Breed Rev. 12: 19-45.
- Asokan R, Mahadeva Swamy HM, Birah A, Geetha G Thimmegowda. 2013. Bacillus thuringiensis Isolates from Great Nicobar Islands. Curr Microbiol. 66: 621626.
- Baig DN, Mehnaz S. 2010. Determination and distribution of cry-type genes in halophilc Bacillus thuringiensis isolates of Arabian Sea sedimentary rocks. Microbiol Res. 165(5):376-83.https://doi.org/10.1016/j.micres.2009.08.003 PMid:19850456
- Baig DN, Bukhari DA, Shakoori AR. 2010. Cry genes profiling and the toxicity of isolates of Bacillus thuringiensis from soil samples against American bollworm, Helicoverpa armigera. J Appl Microbiol. 109(6): 1967-1978. https://doi.org/10.1111/j.13652672.2010.04826.x PMid:20738439
- Barloy F, Lecadet M-M, Delécluse A. 1998. Distribution of clostridial cry-like genes among Bacillus thuringiensis and Clostridium strains. Curr Microbiol. 36: 232-237. https://doi.org/10.1007/s002849900300 PMid:9504991
- Ben-Dov E, Zaritsky A, Dahan E, Barak Z, Sinai R, Manasherob R, A Khamraev, E Troitskaya, Dubitsky A, Berezina N, Margalith Y. 1997. Extended screening by PCR for seven cry-group genes from field-collected strains of Bacillus thuringiensis. Appl Environ Microbiol. 63: 4883-90.
- Bourque SN, Valero JR, Mercier J, Lavoie MC, Levesque RC. 1993. Multiplex polymerase chain reaction for detection and differentiation of the microbial insecticide Bacillus thuringiensis. Appl Environ Microbiol. 59:(2): 523-527
- Bravo A, Sarabia S, Lopez L, Ontiveros H, Abarca C, Ortiz A, Soberon M, Quintero, R. 1998. Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection. Appl Environ Microbiol. 64: 4965-4972.
- Chaubey MK. 2011. Combinatorial action of essential oils towards pulse beetle Callosobruchus chinensis Fabricius (Coleoptera: Bruchidae). Int J Agri Res. 6: 511-516. https://doi.org/10.3923/ijar.2011.511.516
- Chen ML, Chen PH, Pang JC, Chia-Wei Lin CW, Chin-Fa HC, Hau-Yang T. 2014. The correlation of the presence and expression levels of cry genes with the insecticidal activitiesagainst Plutella xylostella for Bacillus thuringiensis strains. Toxins 6: 2453-2470. https://doi.org/10.3390/toxins6082453 PMid:25153253 PMCid:PMC4147593
- Ejiofor AO, Johnson T. 2002. Physiological and molecular detection of crystalliferous Bacillus thuringiensis strains from habitats in the South Central United States. J Ind Microbiol Biotechnol. 28: 284-290 https://doi.org/10.1038/sj/jim/7000244 PMid:11986933
- Federiei BA, Luthy P, Ibarra JE. 1990. Parasporal body of Bacillus thuringiensis israelensis: Structure, protein composition and toxicity. p. 349. In: de Barjac H and Sutherland DJ (Eds.). Bacterial control of mosquitoes and black flies. New Brunswick, Rutgers University Press. https://doi.org/10.1007/978-94-011-5967-8_3
- Head G. 2005. Assessing the influence of Bt crops on natural enemies. Second Inter. Symp. on Biol. Control of Arthropods. Davos, Switzerland, Sept., 12-16.
- Hofte H, Whiteley HR. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53: 242-255.
- Ito T, Ikeya T, Sahara K, Bando H. and Shin-ichiro A. 2006. Cloning and expression of two crystal protein genes, Cry30Ba1 and Cry44Aa1, obtained from a highly mosquitocidal strain, Bacillus thuringiensis subsp. entomocidus. Appl Environ Microbiol. 72:(8): 5673-5676. https://doi.org/10.1128/AEM.01894-05 PMid:16885329 PMCid:PMC1538732
- Konecka E, Baranek J, Hrycak A, Kaznowski A. 2012. Insecticidal activity of Bacillus thuringiensis Strains isolated from soil and water. Scientific World J. 2012: 1-5.
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem. 193: 265-75.
- Md. Abdur Rashid, Bhuiyan Al Sazzad, Rowshan Ara Begum, Reza Md. Shahjahan. 2012. Mortality effect of Bt extracts and esterase variability in three stored grain insects: Callosobruchus chinensis, Sitophilus granarius and Tribolium castaneum. Int J Agric Food Sci. 2(4) : 158-163.
- Morris ON, Converse V, Kanagaratnam P, Cote JC. 1998. Isolation, characterization, and culture of Bacillus thuringiensis from soil and dust from grain storage bins and their toxicity for Mamestra configurata (Lepidoptera: Noctuidae). Can Entomol. 130: 515-537. https://doi.org/10.4039/Ent130515-4
- Nazarian A, Jahangiri R, Jouzani GS, Seifinejad A, Soheilivand S, Bagheri O, Keshavarzi M, Alamisaeid K. 2009. Coleopteran-specific and putative novel cry genes in Iranian native Bacillus thuringiensis collection. J Invertebr Pathol. 102: 101-109. https://doi.org/10.1016/j.jip.2009.07.009 PMid:19631215
- Porcar M, Juarez-Perez VP. 2003. PCR-based identification of Bacillus thuringiensis pesticidal crystal genes. FEMS Microbiol Rev. 26(5): 419-32. https:// doi.org/10.1111/j.1574-6976.2003.tb00624.x PMid:12586389
- Quesada-Moraga E, Garcıa-Tovar E, Valverde-Garcıa P, Santiago-Alvarez C. 2004. Isolation, geographical diversity and insecticidal activity of Bacillus thuringiensis from soils in Spain. Microbiol Res. 159:(2004): 59-71. https://doi.org/10.1016/j.micres.2004.01.011 PMid:15160608
- Ramalakshmi A, Udayasuriyan V. 2010. Diversity of Bacillus thuringiensis isolated from Western Ghats of Tamil Nadu State, India. Curr Microbiol. 61(1): 13-8. doi: 10.1007/ s00284-009-9569-6. https://doi.org/10.1007/s00284-009-9569-6 PMid:20033169.
- Rangeshwaran R, Velavan V, Frenita DL, Surabhi Kumari, Shylesha AN, Mohan M, Satendra Kumar and Sivakumar G. 2016. Cloning, expression and bioassay of Vip3A protein from an indigenous Bacillus thuringiensis isolate. J Pure Appl Microbiol. 10(2): 1533-1539
- Salehi Jouzani G, Seifinejad A, Saeedizadeh A, Nazarian A, Yousefloo M, Soheilivand S, Mousivand M, Jahangiri R, Yazdani M, Amiri RM, Akbari S.2008. Molecular detection of nematicidal crystalliferous Bacillus thuringiensis strains of Iran and evaluation of their toxicity on free-living and plant-parasitic nematodes. Can J Microbiol. 54: 812-822. https://doi.org/10.1139/W08-074 PMid:18923549
- Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual. 2nd edition. Cold Spring ndHarbor Laboratory, Cold Spring Harbor, N.Y.
- Santana MA, Moccia-V CC, Gillis AE. 2008. Bacillus thuringiensis improved isolation methodology from soil samples. J Microbiol Methods 75:(2): 357-8. doi: 10.1016/j.mimet.2008.06.008. https://doi.org/10.1016/j.mimet.2008.06.008 PMid:18619500
- Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev. 62: 775-806.
- Thammasittirong A, Attathom T. 2008. PCR-based method for the detection of cry genes in local isolates of Bacillus thuringiensis from Thailand. J Invertebr Pathol. 98: 121-126. https://doi.org/10.1016/j.jip.2008.03.001 PMid:18407288
- Travers RS, Martin PA, Reichelderfer CF. 1987. Selective process for efficient isolation of soil Bacillus spp. Appl Environ Microbiol. 53: 1263-1266.
- Uribe D, Martinez W and Ceron J. 2003. Distribution and diversity of cry genes in native strains of Bacillus thuringiensis obtained from different ecosystems from Colombia. J Invertebr Pathol. 82: 119-127. https://doi.org/10.1016/S0022-2011(02)00195-7
- Van Frankenhuyzen K (2009) Insecticidal activity of Bacillus thuringiensis crystal proteins. J Invertebr Pathol. 101: 1-16. https://doi.org/10.1016/j.jip.2009.02.009 PMid:19269294
- Vidal-Quist JC, Castañera P. and González-Cabrera J. 2009. Diversity of Bacillus thuringiensis strains isolated from citrus orchards in Spain and evaluation of their insecticidal activity against Ceratitis capitata. J Microbiol Biotechnol. 19(8): 749-759.
- Zothansanga, Lalhmachhuani N, Senthil Kumar N, Gurusubramanian G. 2011. PCR pathotyping of native Bacillus thuringiensis from Mizoram, India. Sci Vis. 11(3): 171-176.
- Isolation and Characterization of Indigenous Nucleopolyhedrovirus Infecting Fall Armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in India
Abstract Views :237 |
PDF Views:84
Authors
G. Sivakumar
1,
M. Kannan
2,
S. Ramesh Babu
3,
M. Mohan
1,
M. Sampath Kumar
1,
P. Raveendran
1,
T. Venkatesan
1,
R. Rangeshwaran
1,
Chandish R. Ballal
1,
P. Ram Kumar
1
Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, IN
2 Department of Plant Protection, Horticultural College and Research Institute, Periyakulam, Tamil Nadu Agricultural University, Coimbatore 641 003, IN
3 Agricultural Research Station, Borwat Farm, MPUAT, Banswara 327 001, IN
1 ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, IN
2 Department of Plant Protection, Horticultural College and Research Institute, Periyakulam, Tamil Nadu Agricultural University, Coimbatore 641 003, IN
3 Agricultural Research Station, Borwat Farm, MPUAT, Banswara 327 001, IN
Source
Current Science, Vol 119, No 5 (2020), Pagination: 860-864Abstract
Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is an invasive insect pest of maize in India. Natural occurrence of nucleopolyhedrovirus (NPV) infection on S. frugiperda larvae was recorded in 2018 during surveys conducted in maize fields in Chikkaballapura district of Karnataka, and Coimbatore and Tirupattur districts of Tamil Nadu. A strain of S. frugiperda nucleopolyhedrovirus (SpfrNPV NBAIR1) infecting S. frugiperda was isolated from the diseased larvae; morphological and biological characteristics were studied. Electron microscopic studies showed tetrahedral-shaped SpfrNPV occlusion bodies (OBs) of size 1.64 μm. Dose–mortality bioassays revealed that first, second and third instar larvae were equally susceptible (LC50 3.71–5.02 OBs/mm2) to SpfrNPV infection. A PCR technique for detection of viral DNA in S. frugiperda NPV was developed by employing the polyhedrin gene (polh)-specific primers. The amplicon of 618 bp was amplified, sequenced and NCBI GenBank accession number was obtained (MT422725). Blast analysis revealed that SpfrNPV conserved polh gene sequence matched 100% with the reference GenBank sequence (J04333) from the NCBI database which confirmed the identity of the SpfrNPV.Keywords
Insect Pests, Maize, Nucleopolyhedrovirus, Spodoptera frugiperda.- Natural Occurrence of Entomopathogens on the Invasive Fall Armyworm, Spodoptera Frugiperda (j.e. Smith) in South India
Abstract Views :247 |
PDF Views:82
Authors
G. Sivakumar
1,
M. Mohan
1,
M. Kannan
2,
K. Elango
3,
P. Ram Kumar
1,
T. Venkatesan
1,
R. Rangeshwaran
1,
Mahesh S. Yandigeri
1,
O. Dhanyakumar
1
Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, IN
2 Department of Nanoscience and Technology, Periyakulam, Tamil Nadu Agricultural University, Coimbatore 641 003, IN
3 Horticultural Research Station, Tamil Nadu Agricultural University, Kodaikanal 624 103, IN
1 ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, IN
2 Department of Nanoscience and Technology, Periyakulam, Tamil Nadu Agricultural University, Coimbatore 641 003, IN
3 Horticultural Research Station, Tamil Nadu Agricultural University, Kodaikanal 624 103, IN
Source
Current Science, Vol 120, No 4 (2021), Pagination: 619-621Abstract
No Abstract.References
- Sharanabasappa, D. et al., Pest Manage. Hort. Ecosyst., 2018, 24, 23–29.
- Shylesha, A. N. et al., J. Biol. Control, 2018, 32(3), 145–151.
- Mallapur, C. P., Naik, A. K., Hagari, S. Praveen, T., Patil, R. K. and Lingappa, S., J. Entomol. Zool. Stud., 2018, 6(6), 1062–1067.
- Raghunandan, B. L., Patel, N. M., Dave, H. J. and Mehta, D. M., J. Entomol. Zool. Stud., 2019, 7(2), 1040–1043.
- Sharanabasappa, D., Kalleshwaraswamy, C. M., Poorani, J., Maruthi, M. S., Pavithra, H. B. and Diraviam, J., Fla. Entomol., 2019, 1029(2), 619–623.
- Firake, D. M. and Behere, G. T., Biol. Control, 2020, 148, 104303.
- Sivakumar, G. et al., Curr. Sci., 2020, 119, 5.
- Vellend, M., J. Veg. Sci., 2001, 12, 545– 552.
- Sun, B. D. and Liu, X. Z., Appl. Soil Ecol., 2008, 39(1), 100–108.
- Okrikata, E. and Yusuf, O. A., Int. Biol. Biomed. J. Autumn, 2016, 2, 4.
- Suby, S. B. et al., Curr. Sci., 2020, 119(1), 44–51.
- Prasanna, B. M., Huesing, J. E., Eddy, R. and Peschke, V. M., Fall armyworm in Africa: a guide for integrated pest management, CIMMTY, Mexico, 2018, p.109.
- Sinha, K. K., Choudhary, A. K. and Priyanka Kumari, Entomopathogenic fungi, In Ecofriendly Pest Management for Food Security (ed. Omkar), Elsevier, Academic Press, Cambridge, USA, 2016, pp. 475–505.
- Monnerat, R. G. and Bravo, A., Controle Biol., 2000, 3, 163–200.
- Kepler, R. M., Humber, R. A., Bischoff, J. F. and Rehner, S. A., Mycologia, 2014, 106(4), 811–829.
- Effect of insecticides on predatory assassin bug, Sycanus collaris (Fabricius) (Hemiptera: Reduviidae) .
Abstract Views :165 |
PDF Views:97
Authors
Affiliations
1 Department of Agricultural Entomology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore – 560065, Karnataka, India ., IN
2 Division of Germplasm Conservation and Utilization, ICAR National Bureau of Agricultural Insect Resources, Bangalore – 560024, Karnataka, India ., IN
3 Division of Genomic Resources, ICAR National Bureau of Agricultural Insect Resources, Bangalore – 560024, Karnataka, India ., IN
4 Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore – 560065, Karnataka, India ., IN
1 Department of Agricultural Entomology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore – 560065, Karnataka, India ., IN
2 Division of Germplasm Conservation and Utilization, ICAR National Bureau of Agricultural Insect Resources, Bangalore – 560024, Karnataka, India ., IN
3 Division of Genomic Resources, ICAR National Bureau of Agricultural Insect Resources, Bangalore – 560024, Karnataka, India ., IN
4 Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore – 560065, Karnataka, India ., IN
Source
Journal of Biological Control, Vol 35, No 4 (2021), Pagination: 272 - 276Abstract
T:Six insecticides were evaluated for their toxicity against nymphs and adults of assassin bug, Sycanus collaris (Fab.) through contact and stomach mode. The studies revealed that emamectin benzoate (0.4g/L), chlorantraniliprole (0.25ml/L), flubendiamide (0.25 ml/L) and thiamethoxam (0.25 g/L) were considered as relatively safer insecticides for all the nymphal instars and adults of S. collaris. Fenazaquin (1.25ml/L) and quinalphos (2ml/L) caused higher mortality (85-100%) in all the stages of S. collaris. The higher mortality observed in nymphs and relatively lesser mortality rate of adults indicate that the application of the chemical should not be carried out immediately after the release of nymphal instars of S. collaris and adult releases would be ideal in Integrated Pest Management (IPM).Keywords
Biological control, insecticides, mortality, Sycanus collaris .References
- Curkovic T, Burett G, Araya J. 2007. Evaluation of the insecticide activity of two agricultural detergents against the long-tailed mealybug, Pseudococcus longispinus (Hemiptera:Pseudococcidae), in the laboratory. Agric.Técnica (Chile), 67: 422-430. https://doi.org/10.4067/ S0365-28072007000400010
- Dunbar DM, Lawson DS, White S, Ngo N. Emamectin benzoate: control of the Heliothis complex and impact on beneficial arthropods. 1998. In: Dugger P, Richter D, editors. Beltwide Cotton Conference. San Diego, California, USA.
- Esquivel CJ, Martinez EJ, Baxter R, Trabanino R, Ranger CM, MichelA, CanasLA. 2020. Thiamethoxam differentially impacts the survival of the generalist predators, Orius insidiosus (Hemiptera:Anthocoridae) and Hippodamia convergens (Coleoptera: Coccinellidae), when exposed via the food chain. J.
- Insect Sci, 20(4): 13. https://doi.org/10.1093/jisesa/ ieaa070 PMid:32770249 PMCid:PMC7414795
- Griesinger LM, Evans SC, RypstraAL. 2011. Effects of a glyphosate-based herbicide on mate location in a wolf spider that inhabits agroecosystems.Chemosphere, 84: 1461-1466.https://doi.org/10.1016/j.
- chemosphere.2011.04.044 PMid:21555143
- Gupta B, Rani M, Kumar R, Dureja P. 2011. Decay profile and metabolic pathways of quinalphos in water, soil and plants. Chemosphere, 85: 710-716. https://doi.org/10.1016/j.chemosphere.2011.05.059 PMid:21708396
- Gupta RK, Gani M, Jasrotia P, Srivastava K. 2013.Development of the predator Eocanthecona furcellata on different proportions of nucleopolyhedrovirus infected Spodoptera litura larvae and potential for predator dissemination of virus in the field. Biol. Control. 58(4): 543-552. https://doi.org/10.1007/s10526-013-9515-1
- He Y, Zhao J, Zheng Y, Desneux N, Wu K. 2012. Lethal effect of imidacloprid on the coccinellid predator Serangium japonicum and sublethal effects on predator voracity and on functional response to the whitefly Bemisia tabaci.Ecotoxic, 21: 1291-1300. https://doi.org/10.1007/ s10646-012-0883-6 PMid:22447470
- IOBC (International Organization for Biological and Integrated Control). 2018. Pesticides and beneficial organisms. Retreived from: https://www.iobc-wprs.org/ expert_groups/01_wg_beneficial_organ isms.html
- Jyoti DP, Goud BK, 2008. Safety of organic amendments and microbial pesticides to natural enemies in brinjal ecosystem. Ann. Plant Prot. Sci, 16: 123-127.
- Lahm GP, Cordova D, Barry JD. 2009. New and selective ryanodine receptor activators for insect control. Bioorg. Med. Chem, 17: 4127-4133. https://doi.org/10.1016/j. bmc.2009.01.018 PMid:19186058
- Paul AVP, Thyagarajan KS. Toxicity of pesticides to natural enemies of crop pests in India. 1992. In: David BV, editor. Pest Management and Pesticides: Indian Scenario, Madras, India. Narmrutha Publications.
- Pereira AIA, Francisco SR, Jose CZ. 2005. Susceptibility of Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) to Gamma-cyhalothrin under laboratory conditions. Scientia Agricola, 62(5): 478-482. https:// doi.org/10.1590/S0103-90162005000500012
- Sahayaraj K. 2007. Pest control mechanism of reduviids. Jaipur, India. Oxford Book Company.
- Sheikh AH. 2016. Studies on assassin bug (Reduviidae: Hemiptera:Insecta) fauna of Dumna Nature Park, Jabalpur, Madhya Pradesh. J. Zool. Stud, 3(5): 83-86.
- Shorey MM, Hale LL. 1965. Mass rearing of the larvae of nine noctuid species on a simple artificial medium. J. Econ. Entomol, 58: 522-524. https://doi.org/10.1093/ jee/58.3.522
- Wakamura S. 1988. Rearing of the beet armyworm, Spodoptera exigua (Hubner) (Lepidoptera:Noctuidae),
- on an artificial diet in the laboratory. Japanese J. Appl. Entomol. Zool. 32(4): 329-331. https://doi.org/10.1303/ jjaez.32.329 https://doi.org/10.1303/jjaez.32.329
- Yi NN, Kyi W. 2000. Biological Control Research Centre, Mandalay Division, Singaing Township, Paleik,
- Myanmar. Proceedings of the Annual Research Conference (Agricultural Sciences), Yangon, Myanmar.
- Biocontrol Potential and Molecular Characterization of Lipopeptides Producing Bacillus Subtilis Against Sclerotinia Sclerotiorum
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Authors
S. RUQIYA
1,
H. C. GIRISHA
1,
C. MANJUNATHA
2,
R. RANGESHWARAN
2,
A. KANDAN
2,
G. SIVAKUMAR
2,
M. K. PRASANNA KUMAR
3,
D. PRAMESH
4,
K. T. SHIVAKUMARA
2,
H. S. VENU
2,
S. NANDITHA
2,
K. S. ANKITHA
1,
K. ADITYA
2,
N. AARTHI
2,
S. N. SUSHIL
2
Affiliations
1 Department of Agricultural Microbiology, UAS, GKVK, Bengaluru – 560 065, Karnataka, IN
2 ICAR-National Bureau of Agricultural Insect Resources, Bengaluru – 560024, Karnataka, IN
3 Department of Plant Pathology, UAS, GKVK, Bengaluru – 560065, Karnataka, IN
4 Rice Pathology Laboratory ARS, Gangavathi, UAS Raichur – 584104, Karnataka, IN
1 Department of Agricultural Microbiology, UAS, GKVK, Bengaluru – 560 065, Karnataka, IN
2 ICAR-National Bureau of Agricultural Insect Resources, Bengaluru – 560024, Karnataka, IN
3 Department of Plant Pathology, UAS, GKVK, Bengaluru – 560065, Karnataka, IN
4 Rice Pathology Laboratory ARS, Gangavathi, UAS Raichur – 584104, Karnataka, IN
Source
Journal of Biological Control, Vol 36, No 4 (2022), Pagination: 215-221Abstract
Bacillus subtilis is a Gram-positive and endospore producing bacterium. Limited studies have shown that lipopeptides produced by B. subtilis can be inhibitory to phytopathogens. Sclerotinia sclerotiorum is a plant pathogenic fungus which causes various diseases like cotton rot, watery soft rot, stem rot, crown rot and blossom blight in vegetable crops. The objective of the study was to isolate lipopeptides from B. subtilis and study their inhibitory potential against S. sclerotiorum. So, the B. subtilis isolates were extracted from the collected soils of Western Ghats of India. They were initially characterized through morphological parameters followed by PCR amplification of the 16S rDNA gene and confirmation through BLAST algorithm in NCBI database. The lipopeptides produced by these isolates were tested against S. sclerotiorum. B. subtilis strains were effective against S. sclerotiorum and exhibited 18.33 to 29.5 % inhibition under dual culture bio-assay. The antagonistic activity of lipopeptides extracted from B. subtilis strains showed 21.56 to 88.89 % inhibition of S. sclerotiorum in the lowest to highest concentration of lipopeptide tested and was found to be significantly higher than the control. The present study has shown that B. subtilis strains vary in the production of lipopeptides and some of them could produce lipopeptides that are highly inhibitory to S. sclerotiorum. B. subtilis strain NBAIR BSWG1 showed the highest inhibition for S. sclerotiorum. Lipopeptide based poison food technique and the dual culture bioassay results showed that B. subtilis strain NBAIR BSWG1 has immense potential for use in the biological control of S. sclerotiorum. Further studies are being carried out in formulating the lipopeptides for field application.Keywords
Antimicrobial property, biopesticide, PCR, soft rot of vegetable, Western Ghats.References
- Abriouel, H., Franz, C. M., Omar, N. B. and Galvez, A. 2011. Diversity and applications of Bacillus bacteriocins. FEMS Microbiol Rev, 35: 201-232. https://doi.org/10.1111/j.1574-6976.2010.00244.x
- Biniarz, P., Lukaszewicz, M. and Janek, T. 2017. Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides a review. Crit Rev Biotechnol, 37(3): 393-410. https://doi.org/10.3109/07388551.2016.1163324
- Gao, H., Xu, X., Dai, Y. and He, H. 2016. Isolation, identification and characterization of Bacillus subtilis CF-3, a bacterium from fermented bean curd for controlling postharvest diseases of peach fruit. Food Sci Technol Res, 22(3): 377-385. https://doi.org/10.3136/fstr.22.377
- Gautham, S. A., Shobha, K. S., Onkarappa, R. and Kekuda, T. R. 2012. Isolation, character isation and antimicrobial potential of Streptomyces species from Western Ghats of Karnataka, India Res J Pharm Technol, 5(2): 233-238.
- Hashem, A., Tabassum, B. and Allah, E. F. A. 2019. Bacillus subtilis: a plant growth promoting rhizobacterium that also impacts biotic stress. Saudi J Biol Sci, 26: 1291-1297. https://doi.org/10.1016/j.sjbs.2019.05.004
- RUQIYA et al.
- Jeyaseelan, E. C., Tharmila, S. and Niranjan, K. 2012. Antagonistic activity of Trichoderma spp. and Bacillus spp. against Pythium aphanidermatum isolated from tomato damping off. Arch Appl Sci Res, 4(4): 1623-1627.
- Jiang, J., Gao, L., Bie, X., Lu, Z., Liu, H., Zhang, C., Lu, F. and Zhao, H. 2016. Identification of novel surfactin derivatives from NRPS modification of Bacillus subtilis and its antifungal activity against Fusarium moniliforme. BMC Microbiol, 16: 31. https://doi.org/10.1186/s12866-016-0645-3
- Johnson, J. S., Spakowicz, D. J., Hong, B. Y., Petersen, L. M., Demkowicz, P., Chen, L., Leopold, S. R., Hanson, B. M., Agresta, H. O., Gerstein, M. and Sodergren, E. 2019. Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis. Nature Communications, 10(1): 5029. https://doi.org/10.1038/s41467-019-13036-1
- Kaur, P. K., Joshi, N., Singh, I. P. and Saini, H. S. 2016. Identification of cyclic lipopeptides produced by Bacillus vallismortis R2 and their antifungal activity against Alternaria alternate. J Appl Microbiol, 122: 139-152.
- Kumbar, B., Mahmood, R. and Narasimhappa, N. S. 2017. Identification and molecular diversity analysis of Bacillus subtilis from soils of Western Ghats of Karnataka using 16S rRNA bacterial universal primers. Int J Pure App Biosci, 5(2): 541-548. https://doi.org/10.18782/2320-7051.2721
- Leelasuphakul, W., Hemmanee, P. and Chuenchitt, S. 2008. Growth Inhibitory properties of Bacillus subtilis strains and their metabolites against the green mold pathogen (Penicillium digitatum Sacc.) of citrus fruit. Postharvest Biol Technol, 48(1): 113-121. https://doi.org/10.1016/j.postharvbio.2007.09.024
- Li, X. Y., Mao, Z. C., Wang, Y. H., Wu, Y. X., He, Y. Q. and Long, C. L. 2012. ESI LCMS and MS/MS characterization of antifungal cyclic lipopeptides produced by Bacillus subtilis XF-1. Adv Microbial Physiol, 22(2): 83-93. https://doi.org/10.1159/000338530
- Ma, Y., Kong, Q., Qin, C., Chen, Y., Chen, Y., Lv, R. and Zhou, G. 2016. Identification of lipopeptides in Bacillus megaterium by two-step ultrafiltration and LC–ESI–MS/MS. Amb Express 6(1): 1-15. https://doi.org/10.1186/s13568-016-0252-6
- Mardanova, A. M., Hadieva, G. F., Lutfullin, M. T., Khilyas, I. V., Minnullina, L. F., Gilyazeva, A. G., Bogomolnaya, L. M. and Sharipova, M. R. 2016. Bacillus subtilis strains with antifungal activity against the phytopathogenic fungi. Agric Sci, 8(1): 1-20. https://doi.org/10.4236/as.2017.81001
- Miljkovic, M., Jovanovic, S., O’Connor, P. M., Mirkovic, N., Jovcic, B. and Filipic, B. 2019. Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. PLoS One 14(5): e0216773. https://doi.org/10.1371/journal.pone.0216773
- Penha, R. O., Vandenberghe, L. P., Faulds, C., Soccol, V. T. and Soccol, C. R. 2020. Bacillus lipopeptides as powerful pest control agents for a more sustainable and healthy agriculture: Recent studies and innovations. Planta, 251: 1-5. https://doi.org/10.1007/s00425-020-03357-7
- Perez, R. H., Zendo, T. and Sonomoto, K. 2018. Circular and leaderless bacteriocins: Biosynthesis, mode of action, applications and prospects. Front Microbiol, 9: 2085. https://doi.org/10.3389/fmicb.2018.02085
- Schloss, P. D. and Handelsman, J. 2005. Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Appl Environ Microbiol 71: 1501. https://doi.org/10.1128/AEM.71.3.1501-1506.2005
- Sharma, D., Singh, S. S., Baindara, P., Sharma, S., Khatri, N., Grover, V., Patil, P. B. and Korpole, S. 2020. Surfactin like broad Spectrum antimicrobial lipopeptide co-produced with sublancin from Bacillus subtilis Strain A52: Dual reservoir of bioactives. Front Microbiol, 11: 1167. https://doi.org/10.3389/fmicb.2020.01167
- Sicuia, O. A., Olteanu, V., Ciuca, M., Cîrstea, D. M. and Cornea, C. P. 2011. Characterization of new Bacillus spp. isolates for antifungal properties and biosynthesis of lipopeptides. Sci Papers Ser A Agron, 54: 482-491.
- Yu, X., Ai, C., Xin, L. and Zhou, G. 2011. The siderophore-producing bacterium, Bacillus subtilis CAS15, has a biocontrol effect on Fusarium wilt and promotes the growth of pepper. Eur J Soil Sci, 47(2): 138-145. https://doi.org/10.1016/j.ejsobi.2010.11.001
- Zaccardelli, M., Sorrentino, R., Caputo, M., Scotti, R., De Falco, E. and Pane, C. 2020. Stepwise-selected Bacillus amyloliquefaciens and Bacillus subtilis strains from composted aromatic plant waste able to control soil-borne diseases. Agri, 10(2): 30. https://doi.org/10.3390/agriculture10020030
- Zhang, L. and Sun, C. 2018. Fengycins, cyclic lipopeptides from marine Bacillus subtilis strains, kill the plant-pathogenic fungus Magnaporthe grisea by inducing reactive oxygen species production and chromatin condensation. Appl Environ Microbiol, 84(18). https://doi.org/10.1128/AEM.00445-18